SB225002与顺铂联合应用对口腔鳞癌细胞作用的体外研究

发布时间：2018-04-05 02:34

本文选题：SB　切入点：顺铂　出处：《口腔医学研究》2016年02期

【摘要】：目的:观察SB225002与顺铂联合应用对口腔鳞癌细胞的增殖、凋亡及细胞周期的影响,并探讨其机制。方法:分别采用MTT试验及流式细胞术检测SB225002与顺铂单独或联合应用对体外培养的口腔鳞癌细胞的增殖、凋亡及细胞周期的影响,并进行相关分析。结果:在单独应用时,SB225002可一定浓度范围内显著抑制口腔鳞癌细胞的增殖,促进其凋亡,并诱导细胞发生G2/M期阻滞,作用随浓度升高而增强。SB225002与顺铂联合应用时,联合组对口腔鳞癌细胞的抑制效果明显强于单独用药组,P0.05。在一定浓度范围内,SB225002可显著促进口腔鳞癌细胞的凋亡,并诱导细胞发生G2/M期阻滞,其作用随浓度的升高而增强;而SB225002与顺铂联合应用时,联合组的促调亡及诱导细胞G2/M期阻滞作用均显著强于单独用药组,P0.05。结论:SB225002单独或与顺铂联合应用时,均可显著抑制口腔鳞癌细胞的增殖,促进其凋亡,并诱导细胞发生G2/M期阻滞,两者之间存在协同作用。
[Abstract]:Aim: to investigate the effects of SB225002 combined with cisplatin on the proliferation, apoptosis and cell cycle of oral squamous carcinoma cells.Methods: MTT test and flow cytometry were used to detect the effects of SB225002 and cisplatin alone or in combination on the proliferation, apoptosis and cell cycle of oral squamous carcinoma cells cultured in vitro.Results: SB225002 could significantly inhibit the proliferation of oral squamous carcinoma cells and promote their apoptosis in a certain concentration range, and induce G2 / M phase arrest. The effects of SB225002 combined with cisplatin were enhanced with the increase of concentration.The inhibitory effect of the combined group on oral squamous carcinoma cells was significantly stronger than that of the single drug group (P 0.05).SB225002 can significantly promote apoptosis of oral squamous carcinoma cells and induce G _ 2 / M phase arrest in oral squamous carcinoma cells within a certain concentration range, and the effect of SB225002 can be enhanced with the increase of concentration, and when SB225002 is combined with cisplatin,In combination group, the effects of inducing apoptosis and inducing G _ 2 / M phase arrest were significantly stronger than those of control group (P _ (0.05)).Conclusion either alone or in combination with cisplatin, WSB225002 can significantly inhibit the proliferation of oral squamous carcinoma cells, promote their apoptosis, and induce G _ 2 / M phase arrest. There is a synergistic effect between them.
【作者单位】：江西省信丰县骨伤科医院口腔科;南昌大学第一附属医院口腔颌面外科;
【基金】：国家自然科学基金项目(编号:81060087、81460410) 江西省自然科学基金项目(编号:20151BAB205029)
【分类号】：R739.8

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