戴用压膜保持器对两种龈下致病性细菌影响的研究

发布时间：2018-04-08 21:03

  本文选题：压膜保持器　切入点：牙周健康　出处：《滨州医学院》2014年硕士论文

【摘要】：目的：本课题通过实时荧光定量PCR技术,检测正畸患者戴用压膜保持器前后不同阶段龈下致病性菌群中牙龈卟啉单胞菌和具核梭杆菌构成比,从而定量分析两种牙周致病菌含量变化,从细菌的角度研究压膜保持器对患者牙周微生态的影响,使医生对压膜保持器的卫生维护有更明确的认识,以指导临床工作,优化改进压膜保持器,帮助患者更好地保持矫治效果,维护口腔卫生。方法：选取31名结束固定矫治,年龄在14-16岁的青少年患者。在压膜保持器戴用前、戴用2个月和戴用4个月,分别取测试牙位远中唇侧的龈下菌斑通过实时荧光定量PCR技术,检测正畸患者戴用压膜保持器前后不同阶段龈下致病性菌群中牙龈卟啉单胞菌和具核梭杆菌构成比,利用软件SPSS13.0对实验数据进行统计学处理。结果：在戴用压膜保持器2、4个月时,16龈下菌斑中牙龈卟啉单胞菌和具核梭杆菌的百分含量均较戴用压膜保持器前显著降低(p0.0167)；在戴用压膜保持器2个月时,41龈下菌斑中牙龈卟啉单胞菌和具核梭杆菌的百分含量均较戴用压膜保持器前显著降低(p0.0167)；在戴用压膜保持器4个月时,16龈下菌斑中牙龈卟啉单胞菌的百分含量均较戴用压膜保持器前和戴用压膜保持器2个月显著降低(p0.0167)；其他牙位中龈下菌斑中牙龈卟啉单胞菌和具核梭杆菌的百分含量在三个时段无显著变化(p0.0167)。结论：戴用压膜保持器对特定牙位的牙龈卟啉单胞菌和具核梭杆菌影响较大,戴用压膜保持器阶段较戴用前牙龈卟啉单胞菌和具核梭杆菌的百分含量明显降低。戴用压膜保持器较固定矫治对正畸患者牙周健康影响要小。实时荧光定量PCR技术检测牙周细菌精准有效快捷。
[Abstract]:Objective: to detect the ratio of porphyromonas gingivalis to Clostridium nucleatum in subgingival pathogenicity of orthodontic patients by real-time fluorescence quantitative PCR.Thus, quantitative analysis of the changes of the two periodontal pathogenic bacteria was made, and the influence of the membrane presser on the periodontal microecology of the patients was studied from the point of view of bacteria, so that the doctors could have a clearer understanding of the sanitary maintenance of the membrane pressure-retainer, so as to guide the clinical work.Optimize and improve the pressure-film holder to help patients maintain the effect of orthodontic treatment and maintain oral hygiene.Methods: 31 adolescent patients aged 14-16 years were selected.The subgingival plaque of the distal lip of the tooth was measured by real-time fluorescence quantitative PCR before wearing the membrane holder for 2 months and 4 months respectively.The ratio of porphyromonas gingivalis to Clostridium nucleatum in subgingival pathogenicity of orthodontic patients at different stages before and after wearing membrane retainer was detected. The data were statistically processed by software SPSS13.0.Results: the percentage contents of porphyromonas gingivalis and Clostridium nucleus in the subgingival plaque were significantly lower than those before the wearing of the membrane retainer at 2,4 months, and the percentage of subgingival bacteria 41 in the second month after wearing the membrane retainer was significantly lower than that before the wearing of the membrane retainer.The percentage content of porphyromonas gingivalis and Clostridium rhizoides in plaque was significantly lower than that before wearing membrane retainer, and the percentage content of porphyromonas gingivalis in subgingival plaque was significantly higher than that in subgingival plaque at 4 months after wearing membrane retainer.The percentage contents of porphyromonas gingivalis and Clostridium nucleus in subgingival plaque of other teeth were not significantly changed in the three periods before and after wearing membrane retainer for 2 months, and the percentage content of P. gingivalis in other subgingival plaque showed no significant change (p0.0167).Conclusion: the effect of membrane retainer on Porphyromonas gingivalis and Clostridium rubrum in certain tooth position is greater. The percentage content of porphyromonas gingivalis and Clostridium nucleatum in the stage of wearing membrane retainer is obviously lower than that of the former.Wearing membrane holder had less effect on periodontal health of orthodontic patients than fixed orthodontic treatment.Real-time fluorescence quantitative PCR is accurate, effective and fast in detecting periodontal bacteria.
【学位授予单位】：滨州医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R783.5
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本文编号：1723327