脂多糖诱导成骨细胞MC3T3-E1中MMP-13表达的分子机制研究

发布时间：2018-04-09 04:15

本文选题：脂多糖　切入点：基质金属蛋白酶-　出处：《上海交通大学学报(医学版)》2014年11期

【摘要】：目的探讨脂多糖(LPS)对小鼠成骨细胞MC3T3-E1中基质金属蛋白酶-13(MMP-13)表达的影响及其分子机制。方法采用LPS处理传代培养的小鼠成骨细胞MC3T3-E1,Western blotting检测MC3T3-E1中MMP-13及转录因子C/EBPβ、核因子κB(NF-κB)及其抑制蛋白IκB、肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)的表达;观察使用NF-κB特异性抑制剂Bay11-7082干预及特异性siRNA沉默C/EBPβ、TNF-α和IL-6表达对MC3T3-E1中MMP-13表达的影响。结果 Western blotting检测结果显示:LPS能够诱导MC3T3-E1中MMP-13高表达,促进NF-κB、C/EBPβ、TNF-α和IL-6的表达和活化,抑制IκB表达;Bay11-7082干预及siRNA沉默TNF-α表达能有效抑制MC3T3-E1中TNF-α和MMP-13的表达,而siRNA沉默C/EBPβ和IL-6表达对MC3T3-E1中MMP-13的表达无明显影响。结论 LPS通过NF-κB/TNF-α诱导MMP-13在成骨细胞MC3T3-E1中高表达,这为阐明牙周炎的发病机制奠定了基础。
[Abstract]:Objective to investigate the effect of lipopolysaccharide (LPS) on the expression of matrix metalloproteinase-13 (MMP-13) in mouse osteoblasts (MC3T3-E1) and its molecular mechanism.Methods the expression of MMP-13, C/EBP 尾, NF- 魏 B, its inhibitor I 魏 B, tumor necrosis factor- 伪 -TNF- 伪, interleukin-6 (IL-6) in mouse osteoblasts treated with LPS were detected by Western blotting.To observe the effect of Bay11-7082 intervention and specific siRNA silencing the expression of C/EBP 尾 -TNF- 伪 and IL-6 on the expression of MMP-13 in MC3T3-E1.Results Western blotting assay showed that Western could induce high MMP-13 expression in MC3T3-E1, promote the expression and activation of NF- 魏 B C / EBP 尾 -TNF- 伪 and IL-6, inhibit the expression of I 魏 B and inhibit the expression of TNF- 伪 and MMP-13 in MC3T3-E1, and inhibit the expression of TNF- 伪 and MMP-13 in MC3T3-E1 by inhibiting the expression of TNF- 伪 by Bay11-7082 and silencing the expression of TNF- 伪 by siRNA.However, the silencing of C/EBP 尾 and IL-6 by siRNA had no significant effect on the expression of MMP-13 in MC3T3-E1.Conclusion LPS induces high expression of MMP-13 in osteoblast MC3T3-E1 through NF- 魏 B / TNF- 伪, which lays a foundation for elucidating the pathogenesis of periodontitis.
【作者单位】：舟山市妇幼保健院儿童口腔科;重庆医科大学附属口腔医院牙周黏膜科;
【分类号】：R781.4

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