京尼平预处理对脱矿牙本质胶原机械性能的影响

发布时间：2018-04-10 06:20

本文选题：京尼平　切入点：牙本质　出处：《牙体牙髓牙周病学杂志》2014年08期

【摘要】：目的:探讨生物交联剂京尼平预处理对脱矿牙本质机械性能的影响。方法:制备人新鲜离体牙本质片和牙本质粉,经100 g/L磷酸液脱矿5 h后,分别用含0.05、0.1、0.15 mg/mL京尼平的蒸馏水、乙醇、丙酮液进行预处理;然后采用茚三酮比色法检测牙本质粉中的胶原交联度;用万能测试机检测牙本质片试件的极限拉伸强度。结果:3种溶剂配制的京尼平预处理剂均能浓度依赖性地增加牙本质胶原的交联度(P0.05);相同浓度条件下不同京尼平溶剂组的交联度相比,以乙醇溶剂组最高,丙酮溶剂组次之,水溶剂组最低(P0.05);同时还能浓度依赖性地提高脱矿牙本质的极限拉伸强度(P0.05),但相同京尼平浓度各溶剂组的牙本质极限拉伸强度相比,均无显著性差异(P0.05)。结论:京尼平能够浓度依赖性地提高牙本质胶原的交联度及其极限拉伸强度,以乙醇作为溶剂时效果更佳。
[Abstract]:Objective: to investigate the effect of genipin pretreatment on the mechanical properties of demineralized dentin.Methods: fresh human dentin tablets and dentin powder were prepared. After demineralized with 100g / L phosphoric acid solution for 5 hours, the samples were pretreated with distilled water, ethanol and acetone solution containing 0.05g / L 0.1 mg/mL genipine, respectively.Then the crosslinking degree of collagen in dentin powder was detected by ninhydrin colorimetry, and the ultimate tensile strength of dentin specimen was detected by universal testing machine.Results genipin pretreatment prepared with three solvents could increase the crosslinking degree of dentin collagen in a concentration-dependent manner (P 0.05), and the crosslinking degree of different genipin solvent groups was the highest in the same concentration group, followed by acetone solvent group.The water solvent group had the lowest concentration of P0.05, and could also increase the ultimate tensile strength of the demineralized dentin in a concentration-dependent manner, but there was no significant difference in the ultimate tensile strength of the dentin between the same concentration of genipin and the same concentration of genipine.Conclusion: geniapine can increase the crosslinking degree and ultimate tensile strength of dentin collagen in a concentration-dependent manner, especially when ethanol is used as solvent.
【作者单位】：第四军医大学口腔医院军事口腔医学国家重点实验室特诊科;第四军医大学口腔医院军事口腔医学国家重点实验室儿童口腔科;第四军医大学口腔医院军事口腔医学国家重点实验室修复科;
【基金】：陕西省中医药管理局中医药课题(Lc71)
【分类号】：R781.05

【相似文献】