Wnt信号通路相关基因在颌骨牙源性角化囊性瘤微创功能治疗中调控作用的研究

发布时间：2018-04-10 08:16

本文选题：颌骨　切入点：牙源性角化囊性瘤　出处：《青岛大学》2014年硕士论文

【摘要】：目的：颌骨牙源性角化囊性瘤(KCOT)发现时往往己较巨大,可采用微创的开窗灌洗治疗,最大限度地保留了颌骨的功能,但治疗周期较长,其内在信号调控通路还缺少研究。Wnt信号通路与人类肿瘤的发生发展具有密切关系,其在KCOT及其转归中发挥何种作用尚需深入研究。本研究应用基因芯片,检测颌骨KCOT中Wnt信号通路的相关基因,探讨Wnt信号通路相关基因在颌骨KCOT中发挥的作用,并用Wnt信号通路阻断剂临床治疗KCOT病例,以期缩短临床疗程,并验证Wnt信号通路相关基因在颌骨KCOT微创功能治疗中发挥的调控作用。方法： 1.应用基因芯片,检测KCOT中的Wnt信号通路的相关基因,探讨Wnt信号通路相关基因在颌骨KCOT中发挥的作用。 2.临床诊断为颌骨KCOT的病例,分为实验组与对照组,行全景片与CT检查,开窗取活检,明确病理诊断,并行开窗灌洗治疗。实验组应用Wnt信号通路阻断剂(EGCG)进行囊腔灌洗,对照组应用NS作常规灌洗。将实验组和对照组的病例进行比较,观察阻断Wnt信号通路相关基因的表达对颌骨KCOT微创功能治疗的影响。结果： 1.基因芯片检测发现KCOT中存在Wnt信号通路相关基因的差异表达,其中Wnt分子中的Wnt5a表达上调；Wnt分子受体FZD3表达上调,Wnt信号传导通路中的MAPK10, PRKX基因表达上调,CAMK2A表达下调。 2.实验组应用Wnt信号通路阻断剂(EGCG)开窗灌洗治疗颌骨KCOT,仅需5至7个月囊腔即消退,对照组应用NS常规开窗灌洗治疗颌骨KCOT,则需1.5年至2年。实验组应用Wnt信号通路阻断剂EGCG,使得开窗灌洗治疗颌骨KCOT的临床治疗周期大大缩短。结论： 1.颌骨KCOT中存在Wnt信号通路相关基因的差异表达。 2.Wnt信号通路阻断剂EGCG开窗灌洗治疗颌骨KCOT,大大缩短临床治疗周期。
[Abstract]:Objective:KCOT (Odontogenic keratocystic tumor of jaw) is often found to be very large. It can be treated with minimally invasive fenestration and lavage, and the function of jaw is preserved to the maximum extent, but the treatment period is longer.Wnt signaling pathway is closely related to the occurrence and development of human tumors. The role of Wnt signaling pathway in KCOT and its outcome needs further study.In this study, the gene chip was used to detect the genes related to the Wnt signaling pathway in the jaw KCOT, to explore the role of the Wnt signal pathway related genes in the jaw KCOT, and to use Wnt signal pathway blocker to treat the KCOT cases in order to shorten the clinical course of treatment.The regulatory role of Wnt signaling pathway related genes in KCOT minimally invasive functional therapy of jaw was verified.Methods:1.The gene chip was used to detect the genes related to Wnt signaling pathway in KCOT and to explore the role of Wnt signaling pathway related genes in jaw KCOT.2.The patients diagnosed as KCOT were divided into experimental group and control group. Panoramic film and CT were performed, biopsy was performed, pathological diagnosis was confirmed, and fenestration lavage was performed.The experimental group was treated with Wnt signal pathway blocker (EGCG) and the control group was treated with NS.The effects of blocking Wnt signaling pathway related gene expression on KCOT minimally invasive functional therapy were compared between the experimental group and the control group.Results:1.The differential expression of Wnt signaling pathway related genes was found in KCOT by microarray analysis, in which the expression of Wnt5a in Wnt was up-regulated and the expression of FZD3 was up-regulated in Wnt signaling pathway. The expression of PRKX was up-regulated and the expression of CAMK2A was down-regulated.2.In the experimental group, Wnt signal pathway blocker (EGCG) was used for the treatment of maxillary KCOT. It only took 5 to 7 months for the capsule cavity to dissipate, while for the control group, it took 1.5 years to 2 years to treat the maxillary KCOT with NS routine fenestration lavage.The experimental group was treated with Wnt signal pathway blocker, which shortened the clinical treatment period of KCOT.Conclusion:1.Differential expression of genes related to Wnt signaling pathway exists in jaw KCOT.2.Wnt signal pathway blocker EGCG fenestration in the treatment of jaws KCOT greatly shortened the clinical treatment cycle.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R739.8

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