涎腺腺样囊性癌组织中HPV感染及p53、p16、EGFR、Cdc2蛋白表达与预后的关系

发布时间：2018-04-11 11:34

本文选题：涎腺肿瘤 + 腺样囊性癌　；参考：《临床与实验病理学杂志》2017年04期

【摘要】：目的初步探讨HPV感染及p53、p16、EGFR及Cdc2表达与涎腺腺样囊性癌(adenoid cystic carcinoma,ACC)预后的关系。方法收集76例涎腺ACC手术标本,分别采用PCR-DNA反向点杂交及免疫组化SP法检测肿瘤组织中HPV-DNA及p53、p16、EGFR及Cdc2表达,收集临床资料并随访。Kaplan-Meier模型分析患者生存情况,Log-rank检验比较生存曲线,多因素Cox回归模型进行预后分析。结果涎腺ACC组织中HPV感染率为0(0/76)。p53、p16、EGFR及Cdc2蛋白在肿瘤组织中的阳性率分别为76.3%(58/76)、57.9%(44/76)、60.5%(46/76)及64.5%(49/76)。p53、p16、EGFR及Cdc2蛋白表达与患者性别、年龄、肿瘤部位、TNM分期及病理类型无关(P均0.05)。EGFR阳性患者中位总生存期(overall survival,OS)低于阴性患者(χ~2=19.111,P0.01)。EGFR阳性患者中位无进展生存期(progression-free survival,PFS)低于阴性患者(χ~2=6.621,P0.01)。Cdc2阳性患者中位OS短于阴性患者(χ~2=3.870,P0.05)。Cdc2阳性患者中位PFS低于阴性患者(χ~2=6.755,P0.01)。多因素Cox回归模型分析显示EGFR、Cdc2蛋白表达(RR=13.417、13.075,P均0.001)是涎腺ACC患者预后的独立危险因素。结论涎腺ACC组织中未检测出HPV感染。p53、p16、EGFR及Cdc2蛋白在涎腺ACC组织中呈不同程度表达,p16不能作为涎腺ACC患者HPV感染状态的替代指标。EGFR及Cdc2阳性表达是涎腺ACC患者预后的独立危险因素,临床对EGFR及Cdc2蛋白表达患者应密切随访。
[Abstract]:Objective to investigate the relationship between HPV infection, expression of p53 p16 EGFR and Cdc2 and prognosis of adenoid cystic carcinoma of salivary gland.Methods 76 cases of salivary gland ACC were collected and the expression of HPV-DNA, p53 p16EGFR and Cdc2 in tumor tissues were detected by PCR-DNA reverse dot hybridization and immunohistochemical SP method. The clinical data were collected and the survival curves were analyzed by Kaplan-Meier model.Multivariate Cox regression model was used to analyze the prognosis.The median PFS of the patients with positive CDc2 was lower than that of the patients with negative results (蠂 2: 3.870, P 0.05, P 0.01).Multivariate Cox regression analysis showed that the expression of EGFR r-Cdc2 protein was an independent risk factor for the prognosis of salivary gland ACC patients (P 0.001).Conclusion the expression of HPV infection, p53, p16, EGFR and Cdc2 protein in salivary gland ACC tissues is not detected in salivary gland ACC tissue. The expression of p16 protein in salivary gland ACC tissue can not be used as an alternative marker of HPV infection in salivary gland ACC patients. EGFR and Cdc2 positive expression are independent risk factors for prognosis of ACC patients with salivary gland ACC.Patients with EGFR and Cdc2 protein expression should be closely followed up.
【作者单位】：河北省唐山市协和医院病理科;唐山职业技术学院基础医学部病理学教研室;河北省唐山市人民医院放化疗科;
【分类号】：R739.8

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