腺病毒介导HGF转染脂肪干细胞复合温敏型可注射水凝胶对兔颞下颌关节骨关节病髁突软骨的修复作用

发布时间：2018-04-13 04:17

本文选题：脂肪干细胞 + 腺病毒　；参考：《口腔医学研究》2017年09期

【摘要】：目的:探讨肝细胞生长因子(HGF)转染脂肪干细胞复合水凝胶支架材料对促进兔颞下颌关节炎病理状态微环境中受损软骨修复的意义。方法:培养兔脂肪干细胞,利用腺病毒介导对其进行HGF转染并制备ADSCs/HGF/水凝胶支架材料。兔颞下颌关节上腔为注射区并分为5组,A组为空白对照组,B组为模型组,C组为单纯细胞治疗组,单纯脂肪干细胞腔内注射,D组为干细胞复合材料组,为造模后腔内注射干细胞复合可注射水凝胶支架材料,E组为转染后干细胞复合水凝胶注射。所有材料移植成功后各组动物分别于用药第3、9周后取材,制备标本并行髁突软骨扫描电镜、组织学观察及实时定量PCR检测,采用SPSS17.0软件包对数据进行方差分析及t检验。结果:扫描电镜与组织学观察可见,D、E组软骨病变区改善情况优于B、C组。与模型组比较,实时定量PCR结果显示,E组MMP-13表达与空白组相对持平但低于C、D组,差异有显著性(P0.05);E组TIMP-1表达与空白组相对持平但高于C、D组,差异有显著性(P0.05)。结论:腺病毒介导HGF转染脂肪干细胞复合可注射型水凝胶对兔颞下颌关节骨关节病受损软骨具有明显修复作用。
[Abstract]:Aim: to investigate the effect of HGF- transfected adipose stem cells (ASCs) combined with hydrogel scaffold on the repair of damaged cartilage in rabbit temporomandibular arthritis (TMJ) microenvironment.Methods: rabbit adipose stem cells were cultured and transfected with HGF mediated by adenovirus. ADSCs/HGF/ hydrogel scaffolds were prepared.Rabbit temporomandibular joint (TMJ) was divided into 5 groups: group A as blank control group, group B as model group, group C as simple cell therapy group, and group D as stem cell composite group after intracavitary injection of adipose stem cells.Group E was treated with transfection of stem cells combined with hydrogel injection.After all the materials were transplanted successfully, the animals were collected after the third week of treatment. The specimens were prepared with scanning electron microscope, histological observation and real-time quantitative PCR detection. The data were analyzed by SPSS17.0 software package and t-test.Results: scanning electron microscope and histological observation showed that the improvement of cartilage lesion in DU E group was better than that in BUC group.Compared with the model group, real-time quantitative PCR showed that the expression of MMP-13 in group E was relatively equal to that in the blank group but lower than that in group C D, and the difference was significant (P 0.05). The expression of TIMP-1 in group E was similar to that in group C (P 0.05), but higher than that in group C (D), and the difference was significant (P 0.05).Conclusion: Adenovirus-mediated HGF transfection of adipose stem cells combined with injectable hydrogel can repair the damaged cartilage of temporomandibular joint osteoarthritis in rabbits.
【作者单位】：济宁口腔医院口腔颌面外科;济南市口腔医院口腔颌面外科;上海闵行区牙病防治所口腔颌面外科;
【基金】：国家自然科学基金(编号:81170935)
【分类号】：R782.6

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