牙髓卟啉单胞菌促成骨细胞凋亡的机制初探

发布时间：2018-04-13 12:41

本文选题：牙髓卟啉单胞菌 + 超声提取物　；参考：《牙体牙髓牙周病学杂志》2014年11期

【摘要】：目的:观察牙髓卟啉单胞菌超声提取物对成骨细胞凋亡的促进作用,并初步探讨其相关分子机制。方法:用浓度为1、10、100μg/m L牙髓卟啉单胞菌超声提取物作用于成骨细胞,分别采用Hoechst33258染色法观察细胞的形态变化;比色法检测细胞中的Caspase-3酶活性改变;同时通过Caspase抑制剂阻断实验分析相关成骨细胞的凋亡途径。结果:牙髓卟啉单胞菌超声提取物能使成骨细胞形态出现染色质浓缩、碎裂等典型的凋亡改变,并可显著提高成骨细胞的Caspase-3酶活性,且具有浓度依赖性(P0.05);Caspase-8抑制剂和Caspase-9抑制剂可以部分抑制牙髓卟啉单胞菌提取物对成骨细胞的促凋亡作用,而Caspase-3抑制剂则能完全阻断牙髓卟啉单胞菌对成骨细胞的促凋亡作用。结论:牙髓卟啉单胞菌超声提取物具有促成骨细胞凋亡的作用,细胞内、外凋亡途径都参与了该过程,并且是一种依赖Caspase-3的凋亡通路。
[Abstract]:Aim: to observe the effect of ultrasonic extract of porphyromonas pulposus on osteoblast apoptosis and to explore its molecular mechanism.Methods: osteoblasts were treated with ultrasound extract of porphyromonas pulposus at a concentration of 1 10 100 渭 g / m L. The morphological changes of osteoblasts were observed by Hoechst33258 staining, and the changes of Caspase-3 enzyme activity in cells were detected by colorimetric method.At the same time, the apoptosis pathway of osteoblasts was analyzed by Caspase inhibitor blocking experiment.Results: ultrasonic extract of porphyromonas pulposus could make the osteoblasts appear typical apoptosis changes such as chromatin concentration and fragmentation, and could significantly increase the activity of Caspase-3 in osteoblasts.In a dose-dependent manner, the inhibitor of Caspase-8 and Caspase-9 could partly inhibit the apoptosis of osteoblasts induced by the extract of Porphyromonas pulposus, while the inhibitor of Caspase-3 could completely block the apoptosis of osteoblasts induced by Porphyromonas pulposus.Conclusion: the ultrasonic extract of porphyromonas pulposus can promote the apoptosis of osteoblasts. Both intracellular and extracellular apoptotic pathways are involved in this process, and it is a kind of apoptotic pathway dependent on Caspase-3.
【作者单位】：海南省人民医院口腔科;
【基金】：海南省自然科学基金资助项目(812151)
【分类号】：R780.2

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