低温常压等离子体治疗金黄地鼠颊囊黏膜溃疡的实验研究

发布时间：2018-04-13 14:18

本文选题：等离子体 + 口腔溃疡　；参考：《重庆医科大学》2017年硕士论文

【摘要】：目的:本实验拟通过建立口腔溃疡动物模型,观察低温常压等离子体对金黄地鼠实验性颊囊黏膜溃疡愈合时间及溃疡组织中超氧化物歧化酶(superoxide dismutase,SOD)活性、丙二醛(malondialdehyde,MDA)含量的影响。探讨低温常压等离子体用于口腔溃疡局部治疗的可行性,为可能的临床应用提供参考依据。材料与方法:1.利用氧自由基诱导法建立金黄地鼠口腔溃疡模型,溃疡形成后,观察溃疡形态及大小,作病理切片观察判断建模是否成功。2.56只动物随机分为:第1批建模组(n=48)、第2批建模组(即溃疡模型组,n=4,于第1批动物建模72 h后建模)及正常对照组(n=4,正常饲养,不做任何处理)。其中,第1批建模组又随机均分为:溃疡自然愈合组、药物组、2个单纯通气组(30 s、120 s组)及2个等离体处理组(30 s、120 s组),每组8只。第1批建模动物溃疡形成后,记录溃疡形态及大小,进行相应实验处理,每天1次,持续3天。末次治疗结束24 h后即第2批动物建模72 h后,每组随机选取4只动物测定颊囊黏膜溃疡组织中S0D活力及MDA含量;观察剩余24只动物各组溃疡的愈合时间,待溃疡完全愈合后检测相应部位黏膜组织中S0D活力及MDA含量。结果:1.该方法建模72小时后观察到黏膜溃疡形成,溃疡为圆形或类圆形,直径约0.5-0.6cm,表面淡黄色假膜覆盖,周围可有充血、水肿,镜下病理特点与人的口腔溃疡相似。2.(1)溃疡平均面积(0.216±0.014)cm2,各组间溃疡面积相比无显著差异(P0.05);等离子体组愈合时间明显短于其余各组,30、120 s两个等离子体组溃疡愈合时间相比无显著差异(P0.05)。(2)与正常对照组相比,建模成功后的溃疡模型组颊囊黏膜组织中SOD活性显著降低(P=0.00),MDA含量显著增高(P=0.00)。末次处理24 h后,等离子体组SOD活性和MDA含量与正常对照组没有显著差异(P0.05),但与溃疡模型组及其余各组相比均有显著差异(P=0.00);而30s、120s两个等离子体组间没有显著差异(P0.05)。(3)溃疡完全愈合后各实验组囊黏膜组织中SOD活性和MDA含量之间及其与正常对照组间差异没有显著性。结论:1.实验结果显示:以氧自由基诱导法成功建立口腔溃疡动物模型,所得溃疡临床与病理表现与人类口腔溃疡相似。2.实验结果显示:(1)该方法建模,形成的溃疡大小无显著区别;(2)该方法建模后溃疡形成时,黏膜组织中SOD活性明显降低,MDA含量明显增高。30 s、120s等离子体处理,可使甲基紫晶所致金黄地鼠颊囊黏膜溃疡组织中SOD活性及MDA含量较快回复到正常水平,明显缩短溃疡愈合时间。(3)以该方法建立口腔溃疡动物模型,待溃疡完全愈合后,颊囊粘膜组织中SOD、MDA均能回复到正常水平。
[Abstract]:Aim: to establish an animal model of oral ulcer and to observe the effects of plasma at low temperature and atmospheric pressure on healing time of experimental cheek pouch ulcer and the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) of malondialdehyde (MDA) in experimental cheek pouch mucosa of golden hamsters.To explore the feasibility of hypothermia and atmospheric pressure plasma in the local treatment of oral ulcer, and to provide reference for the possible clinical application.Materials and methods: 1.The oral ulcer model of golden hamsters was established by oxygen free radical induction. After the ulcers were formed, the morphology and size of the ulcers were observed.Pathological sections were made to determine whether the model was successful or not. 2.56 animals were randomly divided into three groups: the first group, the first group, the second group, the ulcer model group, the second group (namely, the ulcer model group, for 72 hours after modeling) and the normal control group, the control group, the control group, for normal feeding.Don't do anything.Among them, the first group was randomly divided into three groups: spontaneous ulcer healing group, drug group, two simple ventilation group (30 s / 120 s) and two iso-isolated treatment groups (30 s / 120 s) with 8 rats in each group.After the ulcer was formed in the first batch of model animals, the ulceration morphology and size were recorded, and the corresponding experimental treatment was carried out once a day for 3 days.24 hours after the end of the last treatment, 72 hours after the second batch of animal models, 4 animals in each group were randomly selected to determine the activity of S0D and the content of MDA in the tissues of buccal pouch mucosa ulcer, and the healing time of ulcer in each group of the remaining 24 animals was observed.The activity of S0D and the content of MDA in mucosal tissue were detected after the ulcer healed completely.The result is 1: 1.The formation of mucous ulcer was observed 72 hours after modeling. The ulcer was round or round in diameter, about 0.5-0.6 cm in diameter. The surface was covered with yellowish pseudomembrane, and there could be congestion and edema around it.The average area of ulcer was 0.216 卤0.014 路cm ~ (2) and there was no significant difference in ulcer area between each group (P < 0.05), and the healing time of plasma group was significantly shorter than that of other groups (30120 s and 30120 s).There was no significant difference between the two groups.The activity of SOD in the buccal pouch mucosa of the ulcer model group was significantly decreased after the establishment of the model.After the last treatment for 24 hours,There was no significant difference in SOD activity and MDA content between the plasma group and the normal control group, but there was significant difference between the plasma group and the ulcer model group and other groups, but there was no significant difference between the two plasma groups in 30 s ~ 120 s.There was no significant difference in SOD activity and MDA content between the experimental group and the normal control group.Conclusion 1.The experimental results showed that the animal model of oral ulcer was successfully established by oxygen free radical induction, and the clinical and pathological manifestations of the ulcer were similar to those of human oral ulcer.The experimental results showed that the SOD activity in mucosal tissue decreased significantly when the ulceration was formed by the method, and the content of MDA in the mucosal tissue increased significantly after the ulcer was formed by the method, and the plasma treatment showed that the content of SOD in the mucosal tissue was significantly increased by plasma treatment for .30 sg / 120 s.The activity of SOD and the content of MDA in golden hamster cheek pouch mucosa ulcer tissue induced by methyl amethyst were quickly returned to normal level, and the healing time of ulcer was shortened obviously.) the animal model of oral ulcer was established by this method, and after the ulcer was completely healed, the animal model of oral ulcer was established.MDA in cheek pouch mucosa could return to normal level.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R781.5

【参考文献】