转录因子RORγτ和FOXP3在口腔扁平苔藓病损组织中的表达及意义

发布时间：2018-04-13 23:03

本文选题：FOXP + RORγτ　；参考：《上海口腔医学》2014年04期

【摘要】：目的:口腔扁平苔藓(oral lichen planus,OLP)是一种发生于口腔黏膜的T淋巴细胞介导的自身免疫性疾病。辅助性T细胞(helper T lymphocytes,Th)在OLP的发病过程中具有重要作用,本研究旨在进一步探索OLP患者局部病损组织中辅助性T细胞亚群Th17细胞和Treg细胞的作用。方法:纳入43例OLP患者和13例健康志愿者。采用实时定量PCR法检测局部病损组织中Th17和Treg细胞的特征性转录因子RORγτ和FOXP3的表达,采用GraphPad Prism 5软件对其表达差异进行统计学分析。结果:OLP局部病损组织中转录因子RORγτ和FOXP3的表达显著高于正常黏膜组织,而且均与OLP的临床分型密切相关。萎缩糜烂型OLP组病损组织中RORγτ/FOXP3比值显著高于网状型OLP组和健康对照组,而网状型OLP组的RORγτ/FOXP3比值虽然高于对照组,但差异无显著性。结论:Th17细胞和Treg细胞均参与OLP的局部免疫反应;同时,Th17/Treg失衡也参与了重症型OLP的致病过程,且表现为Th17细胞优势。
[Abstract]:Objective: oral lichen planus (lichen) is an autoimmune disease mediated by T lymphocytes in oral mucosa.Helper T lymphocytes play an important role in the pathogenesis of OLP. The purpose of this study was to further explore the role of helper T cell subsets Th17 and Treg cells in the local lesions of OLP patients.Methods: 43 patients with OLP and 13 healthy volunteers were included.The expression of ROR 纬蟿 and FOXP3 in Th17 and Treg cells were detected by real-time quantitative PCR, and the difference was analyzed by GraphPad Prism 5 software.Results the expression of transcription factor ROR 纬蟿 and FOXP3 were significantly higher in the local lesions of OLP than in normal mucosa, and they were closely related to the clinical classification of OLP.The ratio of ROR 纬蟿 / FOXP3 in OLP group was significantly higher than that in reticular OLP group and healthy control group, but the ratio of ROR 纬蟿 / FOXP3 in reticular OLP group was higher than that in control group, but there was no significant difference.Conclusion both the Th17 cells and the Treg cells are involved in the local immune response of OLP, and the imbalance of Th17 / Treg is also involved in the pathogenesis of severe OLP, which is characterized by the dominance of Th17 cells.
【作者单位】：华中科技大学同济医学院附属同济医院口腔医学中心;湖北省黄冈市妇幼保健院检验科;
【分类号】：R781.5

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