纯钛微弧氧化—多巴胺—载杜仲提取物复合膜层生物相容性研究

发布时间：2018-04-14 07:36

  本文选题：纯钛 + 微弧氧化　； 参考：《佳木斯大学》2017年硕士论文

【摘要】：目的:为改善纯钛种植体的生物学性能,对其表面进行超声微弧氧化-多巴胺-杜仲提取物复合处理,探讨纯钛微弧氧化载杜仲提取物生物膜层对金黄色葡萄球菌、大肠杆菌及成骨细胞生物相容性的影响,为其临床应用提供参考和依据。方法:纯钛微弧氧化-多巴胺为A组,用以对照。纯钛微弧氧化-多巴胺-载0.003125g/ml、0.00625g/ml、0.009375g/ml杜仲提取物复合处理依次为实验B、C、D组。纯钛微弧氧化-多巴胺-载杜仲提取物复合处理试件与金黄色葡萄球菌、大肠杆菌37℃恒温箱内培养,覆膜法检测抗菌性;采用CCK-8法,SEM,ALP法检测试件的细胞相容性。结果:C、D两组具有较强的抗菌性。CCK-8与ALP分析表明,实验组试件表面相比对照组更有利于细胞的粘附与增殖,顺序为CDBA,四组均有统计学意义。激光共聚焦细胞骨架形态学显示C、D两组更利于细胞的铺展和交联。结论:四组材料均有良好的生物相容性,且C组纯钛微弧氧化-多巴胺-0.00625g/ml杜仲提取物生物相容性最佳。
[Abstract]:Objective: to improve the biological properties of pure titanium implants, the surface of the implants was treated with ultrasonic micro-arc oxidation and dopamine-Eucommia ulmoides extract, and the biofilm of pure titanium microarc oxidation containing eucommia ulmoides extract was studied for staphylococcus aureus.The effect of Escherichia coli and osteoblast biocompatibility provides reference and basis for its clinical application.Methods: pure titanium microarc oxidation-dopamine was used as control group.Pure titanium microarc oxidation-dopamine loaded 0.003125g / ml 0.00625g / ml 0.009375g / ml Eucommia ulmoides extract compound treatment followed by the experimental group BCU D.Pure titanium microarc oxidation-dopamine-loaded Eucommia ulmoides extract was cultured with Staphylococcus aureus and incubated in E. coli at 37 鈩，

本文编号：1748316