新消感预防及治疗牙釉质脱矿的体外研究

发布时间：2018-04-14 15:16

本文选题：牙酸蚀症 + 新消感　；参考：《山东大学》2014年硕士论文

【摘要】：1、通过体外实验,研究新消感预防果汁引起的牙釉质脱矿。为酸蚀症的预防提供实验依据。 2、通过体外实验,研究新消感治疗果汁引起的牙釉质脱矿,不同浓度的新消感对硬度改变的影响。为酸蚀症的治疗及临床上使用新消感提供实验据。收集口腔外科拔除的牙冠完整的新鲜上颌第三磨牙40颗,制成牙釉质样本,分为两组实验：①新消感对预防牙齿酸蚀症的体外实验研究：取30个牙釉质样本,随机分三组(对照组、果汁饮料组、果汁饮料+新消感组)。处理7天后于扫描电镜观察各组牙齿牙釉质表面的形态学变化。②新消感对治疗牙齿酸蚀症的体外实验研究：另选取50个牙釉质样本,随机分5组(对照组、25%新消感组、50%新消感组、75%新消感组、100%新消感组),果汁酸蚀7天,然后用不同浓度新消感处理7天,分别测量再矿化实验前后各组样本牙釉质表面显微硬度。 1、新消感预防牙齿酸蚀症实验的扫描电镜观察结果：酸蚀后牙釉质表面形态不规则,呈凸凹不平的粗糙面。新消感加橙汁处理后,散在的凹陷性缺损,表面伴有大量的分布不均匀矿物质颗粒沉积,成块状、片状的沉积物覆盖在釉质表面的凹陷窝内,使脱矿区的深度变浅。 2、新消感治疗牙齿酸蚀症实验的牙釉质表面显微硬度测试结果：再矿化后的对照组(蒸馏水组)及25%新消感组的牙釉质表面显微硬度较再矿化前均无明显上升,差异无统计学意义(P0.05)；50%新消感组、75%新消感组、100%新消感组经再矿化处理后,3组牙釉质表面显微硬度均较再矿化前明显上升,差异有统计学意义(P0.05)。对照组(蒸馏水组)与25%新消感组、50%新消感组与75%新消感组、50%新消感组与100%新消感组、75%新消感组与100%新消感组每两组间的再矿化后的牙釉质表面显微硬度差异无统计学意义(P0.05)；对照组(蒸馏水组)分别与50%新消感组、75%新消感组、100%新消感组的再矿化后的牙釉质表面显微硬度差异均有统计学意义(P0.05)。25%新消感组分别与50%新消感组、75%新消感组、100%新消感组的再矿化后的牙釉质表面显微硬度差异均有统计学意义(P0.05)。 [实验结论] 新消感对牙齿酸蚀症的发展有抑制作用。新消感对已经形成酸蚀症的牙齿有促进再矿化的作用,其作用与浓度有关：25%的新消感对酸蚀症的牙齿无再矿化的作用；50%、75%、100%的新消感对酸蚀症的牙齿均有促进其再矿化的作用。
[Abstract]:1. Through in vitro experiments, the prevention of tooth enamel demineralization caused by juice was studied.To provide experimental basis for the prevention of acidosis.2. Through in vitro experiments, the effects of different concentrations of new desensitization on the hardness of tooth enamel induced by juice were studied.To provide experimental evidence for the treatment of acidosis and clinical use of new Xiaoxiang.A total of 40 fresh maxillary third molars with intact crown extracted by oral surgery were collected and made into enamel samples, which were divided into two groups: 30 enamel samples were collected to prevent tooth acidosis.The rats were randomly divided into three groups (control group, juice drink group, juice drink Xinxiao group).After 7 days of treatment, the morphological changes of enamel surface in each group were observed by scanning electron microscope.They were randomly divided into 5 groups (control group, 25% Xinxiao group, 50% Xinxiaoxen group, 75% Xinxian group, 100% Xinxiao group, 7 days fruit juice acid etching, and then treated with different concentrations of new elimination sensation for 7 days, respectively). The surface microhardness of enamel in each group was measured before and after the remineralization experiment.1. The results of scanning electron microscope (SEM) observation on the prevention of dental acidosis by Xinxiaoxen:The surface of enamel was irregular and rough after acid etching.After the treatment of Xinxiaogan and orange juice, the scattered sunken defect, accompanied by a large amount of uneven distribution of mineral particles deposition, block, sheet sediment covered in the lacuna of enamel surface, make the depth of demineralization shallower.2.The microhardness of enamel surface in the treatment of tooth acidosis:The surface microhardness of enamel in the control group (distilled water group) and 25% Xinxiao group after remineralization showed no significant increase compared with that before remineralization.There was no significant difference in the surface microhardness of enamel between the three groups (P 0.05%, P 0.05%, P 0.05), and the surface microhardness of tooth enamel in group C (P 0.05) was significantly higher than that in group C (P 0.05) after the treatment of remineralization, and the surface microhardness of enamel in group C was significantly higher than that in group C (P 0.05).Control group (distilled water group), 25% Xinxiao group, 50% Xinxian group and 75% Xinxiaoxen group, 50% Xinxian group and 100% Xinxiaoxen group, 75% Xinxiaoxen group and 100% Xinxiaoxen group have no difference in the surface microhardness of enamel after re-mineralization between each two groups, and there is no difference in the microhardness of enamel surface between each of the two groups.There were significant differences in surface microhardness of enamel between the control group (distilled water group) and 50% Xinxiao group (75% Xinxiao group, 100% Xinxiao group) and 50% Xinxiao group (P 0.05% .25% Xinxiao group respectively), while the surface microhardness of enamel surface in the control group (distilled water group) was significantly different from that in the 50% Xinxiao group (100% Xinxiao group).There were significant differences in the surface microhardness of enamel of 75% Xinxiao group (100% XXG) and 100% XXG group (P 0.05).[experimental conclusions]The development of tooth acidosis can be inhibited by new Xiaogan.The effect of Xinxiao on the teeth that have already formed acidosis is related to the concentration. The effect of Xinxiao of 25% on the non-mineralization of the teeth of acidosis. 50% of Xinxiao has the effect of promoting the remineralization of the teeth of acidosis.
【学位授予单位】：山东大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R781

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