阿司匹林对兔颊VX-2鳞状细胞癌炎症微环境中树突细胞的影响

发布时间：2018-04-16 19:57

本文选题：阿司匹林 + 炎症　；参考：《华西口腔医学杂志》2016年02期

【摘要】：目的探索阿司匹林及炎症对兔颊VX-2鳞状细胞癌上清液中树突细胞(DC)成熟及功能的影响。方法通过瘤粒植入术及机械创伤+高糖饮食的方法,建立兔颊VX-2鳞状细胞癌及其炎症模型,将模型兔分为3组。实验组:制造颊癌及炎症后使用阿司匹林灌胃治疗3 d;对照组:制造颊癌及炎症后以生理盐水灌胃作为对照;空白组:不制造炎症的单纯肿瘤兔组。3 d后收集各组肿瘤标本,制成匀浆,取上清液与正常兔外周血单核细胞共培养以使其向DC分化,采用流式细胞仪检测DC表面分子CD83、CD86、人类白细胞DR抗原(HLA-DR)的表达,混合淋巴细胞反应检测DC刺激T细胞增殖的能力。结果实验组和对照组中CD83、CD86、HLA-DR阳性率均低于空白组(P0.05),而实验组和对照组间的差异无统计学意义(P0.05);同样,实验组和对照组刺激T细胞增殖的能力弱于空白组(P0.05),而两组间刺激T细胞增殖的能力无明显差异(P0.05)。结论炎症可抑制DC表面分子CD83、CD86、HLA-DR的表达和功能发挥,短期、小剂量服用阿司匹林对兔颊VX-2鳞状细胞癌炎症微环境中DC的表型改善和功能发挥无明显作用。
[Abstract]:Objective to investigate the effects of aspirin and inflammation on the maturation and function of dendritic cells in the supernatant of rabbit buccal VX-2 squamous cell carcinoma.Methods the rabbit model of VX-2 squamous cell carcinoma and its inflammation was established by means of tumor granulation implantation and high glucose diet after mechanical trauma. The model rabbits were divided into 3 groups.Experimental group: buccal carcinoma and inflammation were treated with aspirin for 3 days; control group: buccal carcinoma and inflammation were treated with normal saline as control group; blank group: rabbit group without inflammation was treated with aspirin for 3 days.The supernatant was cocultured with normal rabbit peripheral blood monocytes to differentiate into DC. Flow cytometry was used to detect the expression of CD83 CD86, HLA-DR antigen of human leukocyte.The ability of DC to stimulate T cell proliferation was detected by mixed lymphocyte reaction.Results the positive rate of HLA-DR in the experimental group and the control group was lower than that in the blank group, but the difference between the experimental group and the control group was not statistically significant (P 0.05).The ability of stimulating T cell proliferation in the experimental group and the control group was weaker than that in the blank group, but there was no significant difference between the two groups in stimulating T cell proliferation.Conclusion inflammation can inhibit the expression and function of HLA-DR on DC surface molecule CD83 and CD86. In short term, low dose aspirin has no effect on the phenotypic improvement and function of DC in the inflammatory microenvironment of rabbit buccal VX-2 squamous cell carcinoma.
【作者单位】：贵州省人民医院口腔科;遵义医学院附属口腔医院口腔颌面外科;
【基金】：遵义市科技局科研基金[遵市科合社字(2013)32号]~~
【分类号】：R739.8

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