慢性心理应激致大鼠咬肌及髁突超微结构损伤后自然恢复的研究

发布时间：2018-04-19 06:43

本文选题：慢性不可预见性轻度应激 + 抑郁　；参考：《牙体牙髓牙周病学杂志》2014年02期

【摘要】：目的:探讨心理应激对髁突和咬肌的影响程度及是否可逆。方法:取8周龄SPF级雄性SD大鼠42只,随机等分为空白对照组、应激后即刻组以及应激后恢复2、4、8、12周共6组(n=7),空白对照组大鼠不予刺激,其他5组大鼠采用慢性轻度不可预见性应激(chronic unpredictable mild stress,CUMS)的方法连续处理12周,建立大鼠抑郁模型;通过体质量、旷场实验、摄糖实验评估大鼠的抑郁程度,采用酶联免疫法(ELISA)检测各大鼠的血清皮质醇(CORT)含量,电镜观察各组大鼠髁突表面和咬肌的超微结构变化。结果:12周的慢性应激后大鼠的血清学、行为学和体质量均发生明显变化(P0.05);大鼠血清CORT浓度、旷场实验及糖水偏爱度分别在去除应激后4、8、12周时恢复正常,但体质量仍然低于对照组(P0.05);12周应激后大鼠髁突与咬肌均出现损伤,去除应激12周后,咬肌基本恢复,髁突表面逐渐恢复但不完全。结论:CUMS导致的大鼠抑郁样行为以及咬肌和髁突软骨的损伤变化在去除CUMS刺激后是可以自然恢复的。
[Abstract]:Objective: to investigate the effect of psychological stress on condyle and masseter muscle.Methods: Forty-two 8-week-old SPF male SD rats were randomly divided into 6 groups (n = 6) in the control group.The other five groups of rats were treated with chronic unpredictable mild stress for 12 weeks in order to establish a depression model, and the depression degree was evaluated by body mass, open field test and glucose uptake test.The content of Cort in serum of rats was detected by Elisa. The surface of condyle and the ultrastructure of masseter muscle in each group were observed by electron microscope.Results there were significant changes in serum, behavior and body mass of rats after 12 weeks of chronic stress, serum CORT concentration, open field test and preference degree of sugar water returned to normal at 812 weeks after removal of stress.However, the body mass was still lower than that in the control group after 12 weeks of stress, both the condyle and masseter muscle were damaged. After 12 weeks of stress, the masseter muscle recovered basically, and the surface of the condyle gradually recovered but not completely.Conclusion the depressive behavior and damage changes of masseter muscle and condylar cartilage induced by the CUMS in rats can be recovered naturally after removal of CUMS stimulation.
【作者单位】：第四军医大学附属口腔医学院;
【基金】：国家自然科学基金资助项目(81100772)
【分类号】：R782.6

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