丝线结扎及局部涂抹牙龈卟啉单胞菌对小鼠牙槽骨骨吸收的影响

发布时间：2018-04-22 15:58

本文选题：牙龈卟啉单胞菌 + 小鼠　；参考：《北京大学学报(医学版)》2017年01期

【摘要】：目的:比较丝线结扎和涂抹牙龈卟啉单胞菌诱导小鼠牙周炎模型的牙槽骨骨吸收程度、激活破骨细胞及去除诱因后成骨的过程。方法:48只C57BL6小鼠随机分配,采用分口设计,根据涂抹2%(质量分数)羧甲基纤维素(carboxymethylcellulose,CMC)或10~9个菌落形成单位(colony-forming units,CFU)的牙龈卟啉单胞菌以及是否丝线结扎分为4组(n=24),分别为对照组(右上第二磨牙单纯涂抹CMC)、丝线结扎组(围绕左上第二磨牙结扎9-0丝线)、涂菌组(右上第二磨牙单纯涂抹牙龈卟啉单胞菌)和丝线结扎+涂菌组(结扎左上第二磨牙并涂抹牙龈卟啉单胞菌),测量釉牙骨质界至牙槽骨嵴顶(cementoenamel junction to the alveolar bone crest,CEJ-ABC)的距离,监测牙槽骨的吸收。48只C57BL6小鼠设计和分组同上,牙槽骨骨面的破骨细胞采用抗酒石酸酸性磷酸酶(tartrateresistant acid phosphatase,TRAP)染色并计数。36只C57BL6小鼠随机分配,其中30只小鼠在左侧上颌第二磨牙结扎9-0丝线,观察3周,有12只小鼠在丝线结扎1周后拆除丝线,继续观察2周,分别在丝线结扎1、2、3周以及丝线拆除1周和2周共5个时间点(n=6)各处死6只小鼠,检测CEJ-ABC距离;未行丝线结扎的小鼠CEJ-ABC距离(n=6)为基线水平。采用单因素方差分析(ANOVA)法对所有数据进行统计学分析。结果:丝线结扎组的小鼠牙周炎,在3、6、9和12周后的CEJ-ABC距离分别为(0.16±0.04)mm、(0.16±0.02)mm、(0.18±0.03)mm、(0.17±0.02)mm,与对照组[(0.09±0.03)mm、(0.10±0.01)mm、(0.12±0.04)mm、(0.12±0.01)mm]和涂菌组[(0.09±0.03)mm、(0.12±0.01)mm、(0.12±0.02)mm、(0.10±0.01)mm]相比差异有统计学意义(P0.05)。与对照组[(0.09±0.03)mm]相比,丝线结扎组第3周的CEJ-ABC距离为(0.16±0.04)mm,比涂菌组[(0.09±0.03)mm]诱导的牙槽骨骨吸收更迅速,丝线结扎和涂抹牙龈卟啉单胞菌结合并未进一步增加牙槽骨的吸收破坏。在丝线结扎1 d后,牙槽骨表面的破骨细胞即被激活,与对照组[(2±2)个]相比在第3天达到高峰[(12±4)个,P0.01]。丝线结扎诱导的小鼠牙周炎,在丝线去除2周后CEJ-ABC距离为(0.07±0.02)mm,与去除丝线前[(0.13±0.01)mm]相比显著降低,提示有显著的牙槽骨再生(P0.01)。结论:丝线结扎术是一种迅速且有效的诱导小鼠牙周炎牙槽骨骨吸收的方式,其诱导的破骨细胞活化在丝线结扎后24 h内发生,3 d达到高峰,去除实验性牙周炎的诱导因素即去除结扎的丝线有助于牙槽骨骨再生。
[Abstract]:Aim: to compare the degree of alveolar bone resorption, the activation of osteoclasts and the osteogenesis after removing the inducement of porphyromonas gingivalis in mouse periodontitis model induced by ligation of filaments and smear of porphyromonas gingivalis. Methods A total of 48 C57BL6 mice were randomly assigned. Porphyromonas gingivalis smeared with carboxymethylcellulose carboxymethylcellulosec (CMCC) or 109 colony forming units (CFU) and whether or not filaments were ligated were divided into 4 groups: control group (right second molars coated with CMCC alone, filamentous knot). Group B (ligation of 9-0 filaments around left second molar, smear group (right upper second molar) and filaments ligation group (ligation of left upper second molar and smear of porphyromonas gingivalis) were measured. The distance between the cementum boundary of the enamel and the crest of the alveolar crest, the distance between enamel junction to the alveolar bone crestCase (ABJ-ABC), The absorption of alveolar bone was monitored in 48 C57BL6 mice. The osteoclasts on the alveolar bone surface were stained with tartrateresistant acid phosphataseTRAPAPs and counted. 36 C57BL6 mice were randomly assigned. The 9-0 thread was ligated in the left maxillary second molar in 30 mice. For 3 weeks, 12 mice removed the silk thread after 1 week of ligation and continued to observe for 2 weeks. Six mice were killed at 3 weeks after ligation of silk thread and 1 week and 2 weeks after removal of silk thread. The CEJ-ABC distance was detected in each group, and the CEJ-ABC distance of mice without ligation of silk thread was determined as baseline level. All the data were statistically analyzed by ANOVA (single factor ANOVA) method. Compared with the control group [0.09 卤0.03)mm], the CEJ-ABC distance of the filaments ligation group was 0.16 卤0.04mm at the 3rd week, which was faster than the alveolar bone resorption induced by the smear bacteria group [0.09 卤0.03)mm], and the absorption damage of alveolar bone was not further increased by the ligation of filaments and the application of porphyromonas gingivalis. The osteoclasts on the surface of alveolar bone were activated 1 day after the ligation of the filaments, and reached a peak on the 3rd day compared with the control group [2 卤2] [12 卤4] (P0.01). The CEJ-ABC distance of periodontitis induced by ligation of filaments in mice was 0.07 卤0.02mm. the distance of CEJ-ABC was 0.07 卤0.02mm. it was significantly lower than that before filamentral ligation [0.13 卤0.01)mm], indicating that there was significant alveolar bone regeneration (P0.01). Conclusion: the ligation of filaments is a rapid and effective way to induce alveolar bone resorption in mice with periodontitis. The activation of osteoclasts reached its peak within 24 hours after ligation of filaments. Removal of experimental periodontitis induces removal of ligated filaments to facilitate alveolar bone regeneration.
【作者单位】：北京大学口腔医学院·口腔医院牙周科口腔数字化医疗技术和材料国家工程实验室口腔数字医学北京市重点实验室;
【基金】：国家自然科学基金青年基金(81000440) 教育部留学回国人员科研启动基金项目资助~~
【分类号】：R781.4

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