氟对体外培养大鼠成釉细胞活性和细胞凋亡的影响

发布时间：2018-04-23 12:47

本文选题：氟 + 成釉细胞　；参考：《上海口腔医学》2014年05期

【摘要】：目的:观察氟对体外培养的大鼠成釉细胞活性的影响,为探讨氟斑牙的形成机制提供依据。方法 :取对数生长期的大鼠成釉细胞,在培养液中加入浓度为0、0.4、0.8、1.6、3.2、6.4 mmol/L的NaF溶液,培养24、48、72 h后,采用CCK-8检测各组细胞的活性。荧光显微镜下观察成釉细胞核形态的变化,流式细胞术分析氟对细胞凋亡的影响。采用SPSS13.0软件包对数据进行统计学分析。结果:1NaF浓度为0.4、0.8 mmol/L时,对成釉细胞有促增殖作用;NaF浓度为1.6、3.2、6.4 mmol/L时,对成釉细胞的活性有抑制作用,随着NaF浓度的增加,对细胞的抑制作用也逐渐增强,并且这种双向调节作用呈时间依赖性。2NaF浓度为0.4、0.8 mmol/L时,鲜见核破碎;NaF浓度为1.6、3.2 mmol/L时,存在核破碎,并且随着浓度的提高,核破碎的数量随之增加,即1.6 mmol/L浓度的NaF可引起成釉细胞凋亡,随着浓度的增加,细胞凋亡的数量随之增加。结论:1氟对体外培养成釉细胞的增殖具有双向调节作用,即低浓度促进,高浓度抑制。2浓度超过1.6 mmol/L时,NaF诱导成釉细胞凋亡。
[Abstract]:Aim: to observe the effect of fluoride on the activity of rat ameloblasts in vitro and to provide evidence for the formation of dental fluorosis. Methods: rat ameloblast in logarithmic growth stage was cultured for 24 h, and the activity of each group was determined by CCK-8. The morphological changes of ameloblast nucleus were observed under fluorescence microscope and the effect of fluoride on apoptosis was analyzed by flow cytometry. The data were analyzed by SPSS13.0 software package. Results when the concentration of% 1 NAF was 0.40.8 mmol/L, the proliferation of ameloblast was promoted. When the concentration of NAF was 1.6 mmol/L, the activity of ameloblast was inhibited. With the increase of NaF concentration, the inhibitory effect on ameloblast was gradually enhanced. Moreover, when the concentration of NAF was 0.4nA0. 8 mmol/L in a time dependent manner, when the concentration of NAF in nuclear fragmentation was 1. 6N 3. 2 mmol/L, there was nuclear fragmentation, and with the increase of concentration, the number of nucleus fragmentation increased. 1. 6 mmol/L NaF could induce ameloblast apoptosis, and the number of apoptotic cells increased with the increase of NaF concentration. Conclusion the proliferation of ameloblast cultured in vitro can be regulated by 1: 1 fluorine, that is, the low concentration can promote the proliferation of ameloblast, and when the concentration is higher than 1. 6 mmol/L, NaF can induce the apoptosis of ameloblast.
【作者单位】：中国医科大学口腔医学院口腔预防教研室;中国医科大学口腔医学院中心实验室;辽宁省口腔医学研究所;
【基金】：国家自然科学基金(81072245) 辽宁省自然科学基金(20102278)~~
【分类号】：R781.2

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