血链球菌细菌素对牙龈卟啉单胞菌形貌及力学性质的影响

发布时间：2018-04-24 09:42

  本文选题：血链球菌细菌素 + 牙龈卟啉单胞菌　； 参考：《口腔医学研究》2017年08期

【摘要】：目的:研究血链球菌细菌素(简称血链素)对牙龈卟啉单胞菌(Porphyromonas gingivalis,P.g)形貌特征及力学性质的影响。方法:1)复苏、传代并鉴定血链球菌标准菌株ATCC10556、牙龈卟啉单胞菌标准菌株ATCC33277。2)将血链球菌低温高速离心、细胞破碎等方法提取血链素,使之作用于P.g,于37℃厌氧培养48h。3)采用原子力显微镜(AFM)的接触模式,观察血链素作用前后P.g菌体的形貌变化。4)利用AFM测定血链素作用前后P.g的力-距离曲线,并计算黏附力及杨氏模量。结果:1)通过AFM的观察:P.g在血链素的作用下,菌体直径缩小、平均粗糙度及平均峰高度增加(P0.05)。2)通过AFM的观察:血链素作用后,P.g的黏附力、杨氏模量显著降低(P0.001)。正常P.g的黏附力平均值为(0.70±0.10)nN、杨氏模量平均值为(5.54±0.16)MPa;经血链素作用后P.g的黏附力平均值为(0.50±0.10)nN、杨氏模量平均值为(3.97±0.64)MPa。结论:1)血链素可引起牙龈卟啉单胞菌形貌特征发生改变。2)血链素可导致牙龈卟啉单胞菌的黏附力及杨氏模量降低。
[Abstract]:Aim: to study the effect of streptococcus sanguis bacteriocin on the morphology and mechanical properties of Porphyromonas gingivalis P.g. Methods: resuscitation, subculture and identification of Streptococcus sanguis standard strain ATCC 10556, standard strain ATCC 33277.2) the Streptococcus sanguis was centrifuged at low temperature and high speed, and its blood chain was extracted by cell fragmentation. The contact mode of AFM (atomic force microscope) was used to observe the morphologic changes of P.g cells before and after the action of blood chain hormone. (4) the force distance curve of P.g was measured by AFM before and after the action of blood chain hormone, and the contact mode of P.g was observed by atomic force microscope (AFM), which was cultured at 37 鈩，

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