不同炎症状态下牙龈组织中髓样细胞触发性受体-1的表达

发布时间：2018-04-24 14:14

本文选题：髓样细胞触发性受体 + 牙周病　；参考：《福建医科大学学报》2017年05期

【摘要】：目的观察髓样细胞触发性受体-1(TREM-1)在不同炎症状态下牙龈组织中的表达情况,探讨TREM-1在牙周组织疾病发生过程中的可能作用。方法选择自愿接受本研究的牙周健康人群、慢性牙龈炎患者和慢性牙周炎患者各10例。取30例患者的牙龈组织,固定后常规石蜡包埋,制作连续切片,H-E染色行组织学观察,免疫荧光染色观察各组牙龈组织中TREM-1的表达情况。结果 TREM-1定位于细胞膜或细胞质,不同状态牙龈组织的牙龈上皮和结缔组织全层中均有TREM-1表达,其中牙周健康组主要分布于牙龈组织上皮层,尤其集中于牙龈上皮基底层,牙龈组织特异性荧光着色强度不明显;慢性牙龈炎组牙龈组织中特异性荧光着色强度为中等阳性,与牙周健康组比较,阳性细胞数量有所增加;慢性牙周炎组牙龈组织中特异性荧光着色强度为强阳性,与牙周健康组及慢性牙龈炎组比较,阳性细胞数量显著增加。结论 TREM-1在牙龈组织中的表达强度随着牙周组织炎症程度的加重而增强,可能在慢性牙周炎的发生和发展进程中起重要作用。
[Abstract]:Objective to investigate the expression of myeloid cell trigger receptor (TREM-1) in gingival tissues under different inflammatory conditions and to explore the possible role of TREM-1 in the pathogenesis of periodontal diseases. Methods 10 patients with chronic gingivitis and 10 patients with chronic periodontitis were enrolled in this study. The gingival tissues of 30 patients were fixed and embedded in paraffin. The tissue was stained with H-E and the expression of TREM-1 in gingival tissues was observed by immunofluorescence staining. Results TREM-1 was located in the cell membrane or cytoplasm, and TREM-1 was expressed in the gingival epithelium and connective tissue in different states, especially in the basal layer of gingival epithelium in the healthy periodontal group. The intensity of specific fluorescent staining in gingival tissue was not obvious, and the positive intensity was moderate in chronic gingivitis group, and the number of positive cells in gingival tissue was higher than that in healthy gingivitis group. The intensity of specific fluorescent staining in gingival tissue of chronic periodontitis group was strongly positive, and the number of positive cells was significantly increased compared with that of healthy periodontal group and chronic gingivitis group. Conclusion the expression of TREM-1 in gingival tissue increases with the severity of periodontal inflammation, which may play an important role in the occurrence and development of chronic periodontitis.
【作者单位】：福建医科大学附属口腔医院福建省高校口腔医学重点实验室;福建医科大学基础医学院;
【分类号】：R781.4

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