以牙髓干细胞构建组织工程化牙本质牙髓复合体修复牙髓损伤

发布时间：2018-04-24 20:06

本文选题：组织工程 + 干细胞　；参考：《中国组织工程研究》2017年33期

【摘要】：背景:研究证明,牙髓干细胞具有高度增殖、多向分化的干细胞特征,体外实验证明其在一定条件下可诱导分化成多种功能细胞或组织。目前,利用牙髓干细胞构建组织工程化牙本质牙髓复合体移植对牙体缺损进行修复有望成为可能。目的:观察牙髓干细胞构建组织工程化牙本质牙髓复合体移植对大鼠牙体缺损的修复作用。方法:建立去髓牙齿模型大鼠,随机分为模型组及牙髓复合体移植组。建模后,复合体移植组于模型大鼠髓腔中植入牙本质牙髓复合体,模型组大鼠不作处理。移植后3,5,7周采集标本,对大鼠牙齿组织切片苏木精-伊红和免疫荧光染色,采用图像软件系统测量大鼠牙本质厚度。结果与结论:(1)牙髓细胞形态:牙髓细胞贴壁生长,大多数呈成长梭形、椭圆形,部分呈多角形;第3代细胞长梭形,呈纤维样生长,形态均一,免疫组织化学染色后,细胞核呈现为深蓝色,卵圆形,呈梭形着色深细胞为成纤维细胞样细胞,波形丝蛋白染色呈现为阳性;(2)大鼠牙齿组织苏木精-伊红染色:模型组大鼠牙齿牙本质细胞出现空泡状变性,髓腔内可见被破坏牙髓组织和碎屑,残留不规则的组织条梭,髓腔出现大量红细胞聚集和炎细胞,血管明显扩张。移植后7周,牙本质基质样组织层内可见成牙本质细胞成束装排列,原发性牙本质与再生牙本质界限明显;(3)免疫荧光染色:移植后3周,复合体移植组髓腔内细胞数量明显增多(P0.05);移植后5周,髓腔内细胞数量明显大量增加,并分布于髓壁;(4)测定牙本质厚度:与模型组相比,牙本质牙髓复合体移植组在相同时间点大鼠牙本质厚度均明显较大(P0.05),复合体移植组不同时间点牙本质厚度相比均差异有显著性意义(P0.05);(5)结果证实,牙本质牙髓复合体能促进牙本质的再生和修复。
[Abstract]:Background: dental pulp stem cells have the characteristics of high proliferation and multidirectional differentiation. Experiments in vitro have proved that dental pulp stem cells can be induced to differentiate into a variety of functional cells or tissues under certain conditions. At present, it is possible to use dental pulp stem cells to construct tissue engineered dentin pulp complex graft to repair tooth defect. Aim: to investigate the effect of tissue engineered dentin pulp complex transplantation with dental pulp stem cells on the repair of rat dental defect. Methods: the model rats were randomly divided into two groups: model group and dental pulp complex transplantation group. After modeling, the dental pulp complex was implanted into the pulp cavity of the model rats, but the model group was not treated. The rat dentin thickness was measured by image software system after 7 weeks of transplantation and hematoxylin-eosin staining and immunofluorescence staining. Results and conclusion: dental pulp cell morphology: dental pulp cells, most of which grew fusiform, elliptical and polygonal, and the third generation cells were fusiform, fibrous, homogeneous, and stained by immunohistochemistry. The nuclei were dark blue, oval, fusiform stained, fibroblast-like cells. Vimentin staining: hematoxylin-eosin staining in dental tissue of rats: vacuolar denaturation of dentine cells in the model group, destruction of pulp tissue and detritus in the pulp cavity, and irregular tissue shuttles remained in the pulp cavity of the model group, the rat teeth were stained with hematoxylin and eosin, and the teeth of the model group were stained with hematoxylin-eosin. A large number of erythrocyte aggregation and inflammatory cells appeared in the medullary cavity, and the blood vessels were dilated obviously. At 7 weeks after transplantation, dentin cells were bunched in the dentin stromal tissue layer, and the boundary between primary dentin and regenerated dentin was obvious. Immunofluorescence staining was observed at 3 weeks after transplantation. In the complex transplantation group, the number of intramedullary cells increased significantly (P 0.05), and at 5 weeks after transplantation, the number of intramedullary cells increased significantly, and distributed in the pulp wall (4) the thickness of dentin was measured: compared with the model group, there was no significant difference between the two groups. The dentin thickness of the dentine pulp complex group was significantly larger than that of the control group at the same time point (P 0.05), and there were significant differences in the dentin thickness between the two groups at different time points (P 0.05), the results showed that the dentin thickness of the dentine pulp complex transplantation group was significantly higher than that of the control group at the same time point (P 0.05). Dentin pulp complex can promote the regeneration and repair of dentin.
【作者单位】：河南省人民医院口腔科;
【基金】：河南省卫生厅项目(201403153)~~
【分类号】：R78

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