自体BMSCs复合PRF行位点保存中对Notch1与Wnt3a信号分子的实验研究

发布时间：2018-04-26 06:05

本文选题：Notch1 + Wnt3a　；参考：《新疆医科大学》2016年硕士论文

【摘要】：目的:通过建立自体骨髓间充质干细胞复合富血小板纤维蛋白修复兔拔牙窝骨缺损的动物模型,探讨Notch1和Wnt3a信号通路分子在牙槽骨缺损修复过程中的表达及意义。材料及方法:选用36只健康雄性2月龄新西兰兔,随机分为四组,全麻下均微创拔除下颌左侧中切牙。A组植入自体骨髓间充质干细胞与富血小板纤维蛋白复合物,B、C组分别植入单一富血小板纤维蛋白、自体骨髓间充质干细胞,D组无任何材料植入的空白对照。术后4、8、12周(每个时间点3只)处死动物并立即在骨缺损同一部位取材,制作骨标本。利用免疫组化和免疫荧光检测骨缺损修复过程中Notch1和Wnt3a的表达情况。结果:免疫组化显示第4和8周A、B、C组Notch1和Wnt3a表达均大于D组(P0.05)。12周时,D组这两个通路信号分子的表达大于其他三组(P0.05)。免疫荧光说明在骨缺损区新生骨细胞的Notch1和Wnt3a表达第4周时达到高峰,随着时间的延长骨缺损修复完成,表达消失。结论:Notch1和Wnt3a信号分子在自体骨髓间充质干细胞复合富血小板纤维蛋白促进骨缺损修复过程中表达,它们通过调控自体骨髓间充质干细胞的增殖与分化加速牙槽骨缺损修复的愈合过程。并且两者表达相似,有可能存在“串话”。
[Abstract]:Objective: to investigate the expression and significance of Notch1 and Wnt3a signal pathway molecules in alveolar bone defect repair by establishing an animal model of autogenous bone marrow mesenchymal stem cells combined with platelet-rich fibrin. Materials and methods: 36 healthy male 2-month-old New Zealand rabbits were randomly divided into four groups. The left central incisor of the mandible was extracted by minimally invasive extraction under general anesthesia. Group A was implanted with autologous bone marrow mesenchymal stem cells and platelet-rich fibrin complex. Group C was implanted with a single platelet-rich fibrin. Autologous bone marrow mesenchymal stem cells group D without any material implantation blank control. The animals were killed at 4 weeks and 8 weeks after operation (3 at each time point) and bone specimens were made from the same site of bone defect immediately. Immunohistochemistry and immunofluorescence were used to detect the expression of Notch1 and Wnt3a during bone defect repair. Results: the expression of Notch1 and Wnt3a in group C was higher than that in group D at the 4th and 8th week. The expression of signal molecules of these two pathways in group D was higher than that in group D at week 12. Immunofluorescence showed that the expression of Notch1 and Wnt3a reached its peak at the 4th week in the new bone cells in the bone defect area, and the expression disappeared with the completion of the repair of the bone defect. Conclusion the expression of Wnt3a signaling molecules in autologous bone marrow mesenchymal stem cells combined with platelet-rich fibrin promotes the repair of bone defect. They accelerate the healing of alveolar bone defects by regulating the proliferation and differentiation of autologous bone marrow mesenchymal stem cells. And the two expressions are similar, there may be a "crosstalk".
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R782.1

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