地塞米松对人牙周膜细胞白细胞介素6和白细胞介素8表达的调节作用

发布时间：2018-04-26 15:20

本文选题：地塞米松 + 糖皮质激素　；参考：《中国医科大学学报》2017年05期

【摘要】：目的探讨地塞米松(Dex)对人牙周膜细胞(hPDLCs)中白细胞介素(IL)6和IL-8表达的调节作用。方法 Dex或牙龈卟啉单胞菌(P.g)-脂多糖(LPS)分6组处理hPDLCs:10-9mol/L Dex组,10~(-6)mol/L Dex组,10μg/mL P.g-LPS组,10-9mol/L Dex+10μg/mL P.g-LPS组,10~(-6)mol/L Dex+10μg/mL P.g-LPS组,0.1%无水乙醇(对照组)。分别用ELISA法和实时定量PCR法检测IL~(-6)、IL-8蛋白和mRNA表达水平。结果 24 h和48 h时,10-9mol/L Dex组和10~(-6)mol/L Dex组IL~(-6)和IL-8蛋白表达水平显著低于对照组(P0.05)。48 h时10-9mol/L Dex组和10~(-6)mol/L Dex组hPDLCs IL~(-6) mRNA水平显著低于对照组(P0.05);而10~(-6)mol/L Dex组IL-8 mRNA的表达水平显著高于对照组(P0.05)。24 h和48 h时10-9mol/L Dex+10μg/mL P.g-LPS组和10~(-6)mol/L Dex+10μg/mL P.g-LPS组IL~(-6)蛋白和mRNA表达水平显著低于10μg/mL P.g-LPS组(P0.05)。24 h时10-9mol/L Dex+10μg/mL P.g-LPS组和10~(-6)mol/L Dex+10μg/mL P.g-LPS组IL-8蛋白表达水平显著低于10μg/mL P.g-LPS组(P0.05),但48 h未见统计学差异。48 h时,10~(-6)mol/L Dex+10μg/mL P.g-LPS组IL-8 mRNA水平显著高于10μg/mL P.g-LPS组(P0.05)。结论 Dex可以显著抑制hPDLCs固有的和P.g-LPS诱导产生的IL~(-6)的表达,但是Dex对hPDLCs IL-8的表达具有复杂的调节作用,需注意高浓度Dex长时间作用时对hPDLCs IL-8表达的促进作用。
[Abstract]:Objective to investigate the regulatory effect of dexamethasone Dexon on the expression of interleukin-6 and IL-8 in human periodontal ligament cells (hPDLCs). Methods Dex or P. gingivalis P.gingival-lipopolysaccharide (LPS) were divided into 6 groups. The hPDLCs:10-9mol/L Dex group was treated with 10 ~ (-9) mol 路L ~ (-1) Dex and 10 ~ (-9) mol / L Dex _ (10 渭 g/mL P.g-LPS) group with 10 ~ (-9) mol / L Dex _ (10 渭 g/mL P.g-LPS) and 0.1% anhydrous ethanol in 10 ~ (-9) mol 路L ~ (-1) Dex _ (10) 渭 g/mL P.g-LPS group (control group). The expression of IL-8 and mRNA were detected by ELISA and real time quantitative PCR, respectively. Results at 24 h and 48 h after treatment, the expression levels of IL-8 protein and IL-8 protein in 10-9 mol / L Dex group and 10~(-6)mol/L Dex group were significantly lower than those in 10-9mol/L Dex group and 10~(-6)mol/L Dex group at 48 h, and the expression level of IL-8 mRNA in 10~(-6)mol/L Dex group was significantly higher than that in control group. The protein and mRNA expression levels of 10-9mol/L Dex 10 渭 g/mL P.g-LPS and 10~(-6)mol/L Dex 10 渭 g/mL P.g-LPS at 24 h and 48 h in control group were significantly lower than those in 10-9mol/L Dex 10 渭 g/mL P.g-LPS group and 10~(-6)mol/L Dex 10 渭 g/mL P.g-LPS group at 24 h after 10-9mol/L Dex 10 渭 g/mL P.g-LPS and 10~(-6)mol/L Dex 10 渭 g/mL P.g-LPS, but not at 48 h. The level of IL-8 mRNA in 10 mol / L Dex 10 渭 g/mL P.g-LPS group was significantly higher than that in 10 渭 g/mL P.g-LPS group at 48 h. Conclusion Dex can significantly inhibit the expression of hPDLCs inherent and P.g-LPS induced ILP 6), but Dex has a complex regulatory effect on the expression of hPDLCs IL-8. It is necessary to pay attention to the promoting effect of high concentration Dex on hPDLCs IL-8 expression after a long period of time.
【作者单位】：中国医科大学口腔医学院牙周科;
【基金】：中国博士后科学基金(20090461190)
【分类号】：R781.42

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