氯化锂减小正畸性牙齿移动中牙根吸收

发布时间：2018-05-01 18:00

本文选题：氯化锂 + 正畸性牙齿移动　；参考：《吉林大学》2014年硕士论文

【摘要】：目的：检测氯化锂对大鼠正畸牙移动过程中牙根吸收和牙移动的影响，并探讨将Wnt通路作为调控牙周组织改建靶点的可行性。方法：选择鼠龄在8周，体重约200g的成年健康雄性SD大鼠10只进行饲养。将大鼠随机分为实验组和对照组，每组各5只并建立正畸牙移动模型。即在全身麻醉条件下，在上颌中切牙和上颌右侧第一磨牙之间放置镍钛拉簧，以上颌中切牙为支抗拉第一磨牙向近中，弹簧施加的力值为50g。实验组大鼠每2天通过灌胃法按200mg/kg剂量给予LiCl溶液，对照组给予同等剂量的NaCl溶液。在加力14天后脱臼法处死大鼠，分离第一、二磨牙及其牙周组织，修剪后进行后续检测。首先用Micro-CT对组织块进行三维扫描并进行图像重建，随后通过软件分析第一二磨牙牙冠最突点之间的距离；在完成Micro-CT的拍摄后，将组织块进行修整，并在5%的次氯酸钠溶液中浸泡12小时，小心的剥离第一磨牙牙根，并尽量擦去周围软组织。待牙根表面干燥后，，进行扫描电镜拍摄，重点分析远中颊侧根和远中腭侧根，通过Image J软件计算上述两根近中面的吸收面积比率；最后通过spss软件进行统计分析。结果：实验组和对照组大鼠第一磨牙和第二磨牙之间均有间隙出现，实验组大鼠第一磨牙和第二磨牙之间的距离略大于对照组，但是二者并无显著统计学差异（P=0.224）。实验组和对照组大鼠第一磨牙远中腭侧根和远中颊根近中面都观察到了不同程度的牙根吸收。实验组大鼠牙根吸收比率小于对照组，且二者间有显著统计学差异(P0.05)。结论：通过本实验可以得出，氯化锂能够减轻正畸性牙齿移动过程中牙根吸收的严重程度，而对牙齿移动无显著的影响。
[Abstract]:Aim: to investigate the effects of lithium chloride on root resorption and tooth movement during orthodontic tooth movement in rats, and to explore the feasibility of using Wnt pathway as a target for regulating periodontal tissue remodeling. Methods: ten adult male SD rats aged 8 weeks and weighing about 200 g were selected for feeding. Rats were randomly divided into experimental group and control group with 5 rats in each group and established orthodontic tooth movement model. Under general anesthesia, a nickel titanium pull spring was placed between the maxillary central incisor and the right first molar of the maxilla. The maxillary central incisor was used as the Anchorage to pull the first molar to the near center, and the force applied by the spring was 50 g. Rats in the experimental group were given LiCl solution at the dose of 200mg/kg every 2 days, while the rats in the control group were given the same dose of NaCl solution. The first and second molars and their periodontal tissues were isolated and pruned for subsequent detection. First, the tissue block was scanned and reconstructed by Micro-CT, and then the distance between the most protruding points of the first and second molars was analyzed by software. Soaking in 5% sodium hypochlorite solution for 12 hours, carefully stripping the root of the first molar and erasing the surrounding soft tissue as much as possible. After the root surface was dried, scanning electron microscopy was used to analyze the distal buccal lateral root and distal palatal lateral root. The absorptive area ratio of the two proximal midplane was calculated by Image J software. Finally, the statistical analysis was carried out by spss software. Results: there was a gap between the first molar and the second molar in the experimental group and the control group. The distance between the first molar and the second molar in the experimental group was slightly larger than that in the control group, but there was no significant difference between the two groups. Different degrees of root resorption were observed in the distal palatine lateral root and distal buccal root of the first molar in the experimental group and the control group. The ratio of root resorption in the experimental group was lower than that in the control group, and there was a significant difference between the two groups (P 0.05). Conclusion: lithium chloride can reduce the severity of root resorption during orthodontic tooth movement, but has no significant effect on tooth movement.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R783.5

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