超顺磁性支架材料修复兔下颌骨缺损的实验研究

发布时间：2018-05-03 09:26

本文选题：超顺磁性支架材料 + 颌骨缺损　；参考：《医学研究生学报》2017年03期

【摘要】：目的超顺磁性材料在骨组织工程中的研究较少。文中旨在探讨超顺磁性支架材料聚乳酸-羟基乙酸(PLGA)/含铁的羟基磷灰石颗粒(Fe-HA)修复兔下颌骨缺损的成骨效能并评估材料的生物相容性。方法随机选取新西兰大白兔36只,采用随机数字表法分为6组:分别为术后非静磁场饲养条件下的非静磁场对照组(不植入材料),非静磁场PLGA组(植入PLGA膜材料)、非静磁场PLGA/Fe-HA组(植入PLGA/Fe-HA材料),静磁场饲养条件下的静磁场对照组(不植入材料)、静磁场PLGA组(植入PLGA膜材料)、静磁场PLGA/Fe-HA组(植入PLGA/Fe-HA材料),每组6只。制备双侧下颌骨缺损模型,分别于术后4、12周处死实验动物,标记并制备颌骨标本,进行大体观察、Micro CT扫描、HE染色,并对缺损区骨密度值结果进行分析。12周术后实验兔进行血液学检测、肝肾组织进行病理检验。结果非静磁场PLGA/Fe-HA组、静磁场PLGA/Fe-HA组4周时开始出现缺损区边缘粗糙,缺损直径减小,新生骨形成表现;12周时缺损区形成光滑、连续的骨修复。非静磁场PLGA组、静磁场PLGA组12周时表现为粗糙、不整齐的骨痂修复,缺损边缘与正常骨组织边界可见,非静磁场对照组、静磁场对照组缺损中心区域仍有部分骨缺损未修复。12周时与静磁场PLGA/Fe-HA组骨密度值[(572.50±19.09)mg HA/cm3]比较,静磁场PLGA组[(467.00±6.24)mg HA/cm3]、静磁场对照组[(480.67±2.08)mg HA/cm3]、非静磁场PLGA/Fe-HA组[(461.00±19.79)mg HA/cm3]、非静磁场PLGA组[(446.00±11.31)mg HA/cm3]及非静磁场对照组[(422.00±28.28)mg HA/cm3]均明显降低(P0.05)。静磁场PLGA/Fe-HA组4周时骨密度值[(572.50±19.09)mg HA/cm3]较12周的[(276.00±28.28)mg HA/cm3]明显增加(P0.05),其他各组4周与12周组内比较差异均有统计学意义(P0.05)。结论 PLGA/Fe-HA超顺磁性支架材料可促进骨缺损修复,在磁场作用下材料的成骨效能被优化,生物相容性尚可。
[Abstract]:Objective Superparamagnetic materials are rarely studied in bone tissue engineering. The purpose of this study was to investigate the osteogenic effect of superparamagnetic scaffold poly (lactic acid-glycolic acid) PLGAA / Fe-HA containing hydroxyapatite particles in repairing rabbit mandibular defects and to evaluate the biocompatibility of the materials. Methods Thirty-six New Zealand white rabbits were randomly selected. Six groups were randomly divided into six groups: the control group (non-static magnetic field control group), the non-static magnetic field (PLGA) group (implanted PLGA membrane material), the non-static magnetic field PLGA/Fe-HA group (PLGA/Fe-HA material implantation, static magnetic field group), the control group (non-static magnetic field control group), the non-static magnetic field PLGA group (implanted PLGA membrane material), the non-static magnetic field PLGA/Fe-HA group (implanted PLGA/Fe-HA material). Under the condition of magnetic field feeding, the static magnetic field control group (no implanted material), the static magnetic field PLGA group (implanted PLGA membrane material), the magnetostatic field PLGA/Fe-HA group (implanted PLGA/Fe-HA material, 6 rats in each group). The model of bilateral mandibular defect was made. Experimental animals were killed at 4 weeks and 12 weeks after operation. The specimens of jaws were labeled and prepared, and the micro CT scanning and HE staining were observed. The bone mineral density (BMD) of the defect area was analyzed. 12 weeks after operation, the experimental rabbits were examined by hematology, and the liver and kidney tissues were examined by pathology. Results in the non-static magnetic field PLGA/Fe-HA group, the edge of the defect began to appear at 4 weeks in the PLGA/Fe-HA group, the diameter of the defect decreased, and the defect area formed smooth and continuous bone repair at 12 weeks after the appearance of new bone formation. In the non-static magnetic field PLGA group, the static magnetic field PLGA group showed rough, irregular callus repair at 12 weeks, the defect edge was visible with the normal bone tissue boundary, and the non-static magnetic field control group. Bone mineral density (BMD) in the central defect area of the magnetostatic magnetic field control group [572.50 卤19.09)mg HA/cm3] was compared with that in the magnetostatic magnetic field PLGA/Fe-HA group after 12 weeks of unrepaired bone defect. In the PLGA group, 480.67 卤2.08)mg HA/cm3 group, 461.00 卤19.79)mg HA/cm3 group, 446.00 卤11.31)mg HA/cm3 group and 422.00 卤28.28)mg HA/cm3 group, P0.05 was significantly decreased in the PLGA group, the control group [480.67 卤2.08)mg HA/cm3], the non-static magnetic field PLGA/Fe-HA group [461.00 卤19.79)mg HA/cm3], the non-static magnetic PLGA group [446.00 卤11.31)mg HA/cm3] and the non-static magnetic field control group [422.00 卤28.28)mg HA/cm3]. The bone mineral density (BMD) of the PLGA/Fe-HA group was significantly higher than that of the 12th week group [276.00 卤28.28)mg HA/cm3] at the 4th week, and there was significant difference between the other groups in the 4th week and the 12th week group (P 0.05). Conclusion PLGA/Fe-HA superparamagnetic scaffold can promote the repair of bone defect, the osteogenic efficiency of the material is optimized under the action of magnetic field, and the biocompatibility is good.
【作者单位】：南京大学医学院附属口腔医院牙周科;南京大学医学院附属口腔医院牙体牙髓科;南京大学医学院附属口腔医院正畸科;
【基金】：国家自然科学基金(81570952,81300852) 江苏省基金面上项目(BK20161114) 南京市医学科技发展资金资助项目(YKK14109)
【分类号】：R782.4

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