龋齿及牙周病患者与健康人群口腔微生态的比较研究

发布时间：2018-05-04 00:42

本文选题：高通量测序 + 龋齿　；参考：《口腔医学研究》2016年12期

【摘要】：目的:利用高通量测序技术比较健康人和口腔患者口腔微生物的组成和变化。方法:选取典型的龋齿和牙周病患者,收集受刺激的唾液和口咽样本并进行高通量测序,利用生物信息学分析和比较各样品之间的物种组成差异。结果:各组的平均操作分类单元(OTU)为315个,其中健康人口腔内硬壁菌(Firmicutes)、拟杆菌(Bacteroidetes)、蛋白菌(Proteobacteria)、放线杆菌(Actinobacteria)和梭杆菌(Fusobacteria)对应的OTU数目分别73、64、53、19和18个;此外,相对丰富度结果表明硬壁菌(Firmicutes)在儿童组中减少而在青年组和成年组中增多,而蛋白菌(Proteobacteria)在儿童组中增多而在青年组和成年组中减少。结论:年龄段和口腔疾病对牙齿的发育和口腔微生物的组成影响巨大,传统意义上的致病菌可能在口腔健康中同样发挥着维护口腔稳态的作用。
[Abstract]:Objective: to compare the composition and changes of oral microbes between healthy and oral patients by high-throughput sequencing. Methods: typical dental caries and periodontal disease patients were selected and stimulated saliva and oropharynx samples were collected and high-throughput sequencing was performed. The species composition differences were analyzed and compared by bioinformatics. Results: the average operating classification unit (OTU) of each group was 315. The OTU numbers of the three groups were 73n644 / 5319 and 18, respectively, including Firmicutesus, Bacteroidetesus, Proteobacteria, Actinobacteria, Actinobacteria) and Fusobacteria, respectively. The results of relative abundance showed that the number of Firmicuteses decreased in the children and increased in the young and adult groups, while Proteobacteria) increased in the children but decreased in the young and adult. Conclusion: age and oral diseases have great influence on the development of teeth and the composition of oral microbes. The traditional pathogenic bacteria may also play an important role in maintaining oral stability in oral health.
【作者单位】：南昌大学附属口腔医院牙体牙髓科;南昌大学第二临床医学院;南昌大学转化医学研究院;
【基金】：国家自然科学基金地区基金项目(编号:81503364/31560264) 江西省科技厅项目(编号:20151BBG70239)
【分类号】：R781

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