自粘结根管封闭剂的细胞毒性和粘结性能研究

发布时间：2018-05-05 00:33

  本文选题：根管封闭剂 + 细胞毒性　； 参考：《武汉大学》2014年博士论文

【摘要】：第一部分MetaSEAL, RealSeal SE对MC3T3-E1成骨细胞的细胞毒性 目的：评价两种新型自粘结树脂根管封闭剂MetaSEAL和RealSeal SE对MC3T3-E1成骨细胞的细胞毒性,并与传统的环氧树脂根管封闭剂AH plus做对比。 方法：受试各组根管封闭剂注入Teflon管(直径4.8mm,高2mm)中,固化7天后加入培养基浸提。浸提液用培养基倍比稀释为4种浓度：100%、50%、25%、12.5%,MC3T3-E1细胞于其中培养,分别于24h和72h后取出,用CCK-8法检测细胞增殖活性,计算细胞存活率。采用单因素方差分析和Bonferroni多重法,比较在相同的稀释浓度下,不同根管封闭剂的细胞存活率差异；采用t-检验,比较同种浸提液在高浓度(100%、50%)和低浓度(25%、12.5%)下的细胞存活率差异。 结果：MetaSEAL组细胞存活率均显著低于RealSeal SE和AH Plus (p0.05).100%、50%、25%RealSeal SE浸提液的细胞存活率显著低于AH Plus (PO.05),12.5%浓度下存活率与AH Plus相似。AH Plus的细胞存活率在100%、50%浓度时与阴性对照组无差异,在25%、12.5%浓度下均显著高于阴性对照组(P0.05)。RealSeal SE低浓度组的细胞存活率显著高于高浓度组(P0.05),MetaSEAL和AH Plus组的细胞存活率在高低浓度组间无显著差异。 结论：在本实验条件下,MetaSEAL对成骨细胞表现出严重的细胞毒性；RealSealSE在释放物浓度高时毒性强,浓度低时无毒性；而AH Plus基本无毒性。 第二部分MetaSEAL和RealSeal SE与牙根牙本质的推出粘结强度 目的：评价两种新型自粘结树脂根管封闭剂(MetaSEAL, RealSeal SE)与牙根牙本质的粘结强度。 方法：单根管牙36颗,沿颊舌向纵剖为厚度为0.9mm的牙片,在根中部牙本质内制备体积基本相同的模拟根管,5.25%次氯酸钠液、17%EDTA溶液超声冲洗。牙片随机分三组,每组12颗,于模拟根管内分别充填MetaSEAL, RealSeal SE和AH Plus,固化一周。每组取10个牙片20个孔(n=20)进行薄片推出试验。采用Kruskal-Wallis analysis检验和Mann-Whitney检验各组的粘结强度,体视显微镜下观察粘结界面断裂类型和微观形态。剩余2个牙片部分脱矿,用于扫描电镜(SEM)下观察树脂突微观形态。 结果： 1, MetaSEAL的粘结强度(12.01+4.66MPa)显著高于其他组(P0.05); RealSeal SE(5.43±3.68MPa)与AH Plus (7.34±2.83MPa)的粘结强度无显著性差异,MetaSEAL和AH Plus粘结界面断裂的主要类型为混合失败,而RealSeal SE为粘结失败。 2、SEM镜下可见各组样本牙本质壁上均有数量不等的根管封闭剂残留和牙本质小管开口,有的小管内见折断的树脂突。以MetaSEAL组残留的封闭剂和折断的树脂突最多,RealSeal SE组最少。各组牙本质--树脂粘结界面均有裂隙和树脂突形成。MetaSEAL组可见封闭剂大量渗入牙本质小管,形成连续的、长而密集的树脂突；AH Plus组树脂突也较多、较长但不连续；RealSeal SE树脂突最短且最少见。 结论：在完全去除玷污层的情况下,MetaSEAL与牙根牙本质的粘结能力优越,RealSeal SE与AH Plus粘结力相近,在临床可接受范围内。从材料学的角度来说,自粘结树脂根管封闭剂与牙根牙本质的真实粘结性能良好。
[Abstract]:Part one: cytotoxicity of MetaSEAL, RealSeal SE on MC3T3-E1 osteoblasts
Objective: To evaluate the cytotoxicity of two new self bonded resin root canal sealers MetaSEAL and RealSeal SE on MC3T3-E1 osteoblasts and to compare with the traditional epoxy resin root canal sealant AH plus.
Methods: the root canal sealants were injected into the Teflon tube (diameter 4.8mm, high 2mm), and then added to the medium for 7 days. The extract was diluted to 4 concentrations with medium double dilution: 100%, 50%, 25%, 12.5%, and MC3T3-E1 cells were cultured after 24h and 72h respectively. The cell proliferation activity was detected by CCK-8 method and the cell survival rate was calculated. The cell survival rates of different root canal sealants were compared with the single factor ANOVA and Bonferroni multiple methods, and the cell survival rates of the same extract were compared under high concentration (100%, 50%) and low concentration (25%, 12.5%) by t- test.
Results: the cell survival rate of MetaSEAL group was significantly lower than that of RealSeal SE and AH Plus (P0.05).100%, 50%, the cell survival rate of 25%RealSeal SE leaching solution was significantly lower than AH Plus (PO.05). The survival rate of the cells was similar to that of the negative control group at the concentration of 100% and 50%. The survival rate was not different from that of the negative control group at the concentration of 25% and 12.5%. The cell survival rate in the low concentration group of the P0.05.RealSeal SE group was significantly higher than that in the high concentration group (P0.05). There was no significant difference in the cell survival rate between the MetaSEAL and the AH Plus groups in the high and low concentration groups.
Conclusion: in this experimental condition, MetaSEAL showed severe cytotoxicity to osteoblasts, and RealSealSE was toxic when the concentration of the release was high and the concentration was low, while AH Plus was nontoxic.
The second part is the bond strength between MetaSEAL and RealSeal SE and root dentin.
Objective: To evaluate the bond strength between two new self adhesive resin MetaSEAL (RealSeal SE) and root dentin.
Methods: 36 teeth with single root canal, along the cheek and tongue to the thickness of 0.9mm, were divided into 5.25% groups, 5.25% sodium hypochlorite liquid, and 17%EDTA solution ultrasonic irrigation. The teeth were randomly divided into three groups, each group was filled with MetaSEAL, RealSeal SE and AH Plus in each group. Each group was solidified for one week. 10 dental flakes were taken with 20 holes (n=20) to carry out a thin slice test. The bond strength of each group was examined by Kruskal-Wallis analysis test and Mann-Whitney. The fracture type and Micromorphology of the bonding interface were observed under stereoscopic microscope. The remaining 2 teeth were partially demineralized, and the microstructure of the resin process was observed under the scanning electron microscope (SEM).
Result锛，

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