变异链球菌groE操纵子及其表达与调控

发布时间：2018-05-06 04:14

本文选题：变异链球菌 + groE操纵子　；参考：《国际口腔医学杂志》2016年03期

【摘要】：变异链球菌耐受口腔内多种环境的变化,主要依赖于多种热休克蛋白基因的表达。其中,groE操纵子表达的GroES-GroEL蛋白可辅助新合成的以及变性的蛋白质折叠、组装、转运和降解,从而影响细胞的代谢。变异链球菌groE操纵子位于1 834 692~1 832 649位点,包括σ~A型启动子、分子伴侣表达反向重复序列(CIRCE)、groES和groEL及终止子,在进化上高度保守,其表达受热、酸、乙醇和过氧化氢等多种应激环境的诱导,受CtsR和HrcA蛋白的双重负性调节。groE操纵子的调控有Hrc A-CIRCE系统负调控假说和Cts R的负调控两种方式,但其具体调控机制尚未在变异链球菌中得到充分证实。从分子水平上研究变异链球菌groE操纵子的结构和调控机制,有助于进一步阐明细胞的生理过程,为了解细胞在应激和病变状态下的分子调节机制打下基础。
[Abstract]:Streptococcus mutans tolerate changes in various oral environments, mainly dependent on the expression of a variety of heat shock protein genes. Among them, the GroES-GroEL protein expressed by the GroE operon can assist the newly synthesized and denatured proteins to fold, assemble, transport and degrade, thus affecting the metabolism of the cells. The groE operon of Streptococcus mutans is located at 1 834 692 and 1 832 649, including 蟽 A promoter. The molecular chaperones express reverse repeat sequences, CIRCECEGES, groEL and Terminator. They are highly conserved in evolution and express heat and acid. Induced by many stress environments, such as ethanol and hydrogen peroxide, the double negative regulation of CtsR and HrcA protein. Groe operon is regulated by Hrc A-CIRCE system negative regulation hypothesis and Cts R negative regulation mode. However, its specific regulatory mechanism has not been fully confirmed in Streptococcus mutans. To study the structure and regulation mechanism of groE operon of Streptococcus mutans at molecular level is helpful to further elucidate the physiological process of cells and lay a foundation for understanding the molecular regulatory mechanism of cells under stress and pathological conditions.
【作者单位】：吉林大学口腔医院牙体牙髓病科;
【基金】：国家自然科学基金(81170945)~~
【分类号】：R781.1

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