口腔扁平苔藓患者血清中白细胞介素-12和白细胞介素-27表达与免疫功能的相关性

发布时间：2018-05-06 15:44

本文选题：口腔扁平苔藓 + 白细胞介素-　；参考：《华西口腔医学杂志》2016年02期

【摘要】：目的了解口腔扁平苔藓(OLP)患者血清中白细胞介素(IL)-12和IL-27的表达与患者免疫功能状况的相关性,探讨IL-12和IL-27在OLP免疫发病机制中的作用及意义。方法选取对照组健康者20例和OLP组患者30例(其中网纹型15例,糜烂型15例),通过流式细胞术分析OLP患者外周血淋巴细胞亚群CD3+、CD4+、CD8+、CD19+、CD16+56[自然杀伤细胞(NK)]的表达情况,散射比浊法检测患者体液免疫指标免疫球蛋白(Ig)G、Ig A、Ig M、C3、C4的表达情况。采用酶联免疫吸附试验(ELISA)方法检测血清中IL-12和IL-27的表达水平,分析IL-12和IL-27的表达与OLP患者免疫功能状况及临床特征的相关性。结果与实验室标准值相比,OLP患者细胞免疫中CD3+、CD4+、CD8+水平降低,CD19+水平增高(P0.05);体液免疫中Ig M水平增高,C4水平降低(P0.05)。OLP患者血清中IL-12与IL-27表达水平均高于对照组(P0.05)。同时相关分析显示,OLP患者血清中IL-12和IL-27的表达水平呈正相关关系(r=0.912,P0.01)。但是IL-12及IL-27表达水平与OLP体征计分、病程计分等临床特征无相关性(P0.05)。OLP患者中IL-12和IL-27的表达与CD16+56(NK)存在负相关关系(r1=-0.416,P1=0.022;r2=-0.392,P2=0.032),与Ig G存在正相关关系(r1=0.445,P1=0.014;r2=0.549,P2=0.002)。结论 OLP患者主要以细胞免疫功能低下为主,同时伴有一定程度的体液免疫功能的紊乱。IL-12和IL-27的异常高表达可能协同引起且促进了OLP的炎症发生发展,并且通过机体代偿性负反馈作用参与了对炎症性免疫应答的调节,在OLP的发病机制中起到了一定作用。
[Abstract]:Objective to investigate the relationship between the expression of IL-12 and IL-27 in serum of patients with oral lichen planus (OLP) and their immune function, and to explore the role and significance of IL-12 and IL-27 in the pathogenesis of OLP. Methods A total of 20 healthy controls and 30 patients with OLP (including 15 reticular type and 15 erosive type) were selected to analyze the expression of CD1656 in peripheral blood lymphocyte subsets CD3 CD4, CD8, CD19 and CD1656 by flow cytometry in patients with OLP, including 15 patients with reticular pattern and 15 patients with erosive type. The expression of immunoglobulin G (IgG) and Ig (Ig) C _ (3) C _ (3) C _ 4 in patients with humoral immunity were detected by scattering turbidimetry. The expression of IL-12 and IL-27 in serum was detected by Elisa, and the correlation between the expression of IL-12 and IL-27 and the immunological function and clinical characteristics of OLP patients was analyzed. Results compared with the standard value in laboratory, the level of CD3 CD4 and CD8 decreased and the level of CD19 increased in OLP patients, while the level of IgM in humoral immunity increased and the level of C4 decreased. The expression of IL-12 and IL-27 in serum of patients with OLP was higher than that of control group (P 0.05). At the same time, correlation analysis showed that there was a positive correlation between the expression of IL-12 and IL-27 in the serum of OLP patients. However, there was no correlation between the expression of IL-12 and IL-27 and the score of OLP signs. There was no correlation between the expression of IL-12 and IL-27 in the patients with OLP. There was a negative correlation between the expression of IL-12 and IL-27 and CD16 56K. There was a negative correlation between the expression of IL-12 and IL-27 and CD16 56. There was a positive correlation between the expression of IL-12 and IL-27 and the expression of IL-12 and IL-27 in the patients with CD16 56nk. There was a positive correlation between the expression level of IL-12 and IL-27 and the score of OLP signs, and the expression of IL-12 and IL-27 was positively correlated with the clinical characteristics of OLP, and the expression of IL-12 and IL-27 was negatively correlated with CD16 56NK.There was a positive correlation between the expression of IL-12 and IL-27 and the expression of IL-12 and IL-27. Conclusion the abnormal expression of IL-12 and IL-27 in patients with OLP may co-induce and promote the development of OLP inflammation, and the abnormal expression of IL-12 and IL-27 may be mainly due to the hypofunction of cellular immunity and a certain degree of humoral immune dysfunction. And through the compensatory negative feedback, it participated in the regulation of inflammatory immune response, and played a certain role in the pathogenesis of OLP.
【作者单位】：贵州医科大学附属医院口腔内科;
【基金】：国家自然科学基金(81460104)~~
【分类号】：R781.5

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