氦氖激光对兔退变颞下颌关节髁突软骨基质表达的影响

发布时间：2018-05-11 14:34

本文选题：氦氖激光 + 颞下颌关节退变　；参考：《中国康复医学杂志》2017年09期

【摘要】：目的:通过研究氦氖激光对退变颞下颌关节髁突软骨细胞外基质基质胶原2(Col-2)、蛋白多糖4(PRG-4)表达的影响,明确氦氖激光对颞下颌关节退变的保护作用与机制,为临床运用氦氖激光治疗颞下颌关节骨关节病提供依据。方法:将骨骼发育成熟的健康成年雄性新西兰大白兔随机分为正常组、假模型组、模型组和干预组,每组10只,组内又分为造模后第1天、第11天两个取材时间点。参照常嘉等的造模方法,建立颞下颌关节骨关节病动物模型并行CT检查证实造模成功后纳入。干预组采用氦氖激光照射患侧颊车、上关、下关、听宫、翳风穴,5min/穴/d,共10d;假模型组仅作关节囊切开后即缝合组织。各组于不同时间点取出颞下颌关节组织,采用HE染色观察干预前后形态学改变;Western Blot方法检测Col-2、PRG-4的表达水平。结果:退变髁突软骨HE染色可见纤维降解,增殖层及过度层细胞排列紊乱,细胞核出现皱缩,核浓染,经过氦氖激光治疗后的髁突软骨纤维层轻度松解,部分纤维合成,红染增强。Western Blot检测发现正常组及假模型组不同时间点之间Col-2、PRG-4蛋白表达差异无显著性意义;模型组第11天各细胞外基质蛋白表达明显降低;氦氖激光干预10天后各细胞外基质蛋白的表达明显增加。结论:氦氖激光干预可上调退变颞下颌关节软骨细胞外基质Col-2、PRG-4蛋白表达,纠正细胞外基质降解,从而防治颞下颌关节骨关节病。
[Abstract]:Objective: to study the effect of He-Ne laser on the expression of extracellular matrix collagen2 (Col-2G) and proteoglycan 4 (PRG-4) in degenerative chondrocytes of temporomandibular joint (TMJ), and to clarify the protective effect and mechanism of He-Ne laser on the degeneration of temporomandibular joint (TMJ). To provide evidence for clinical treatment of temporomandibular osteoarthropathy with He-Ne laser. Methods: healthy adult New Zealand white rabbits with mature skeletal development were randomly divided into normal group, sham model group, model group and intervention group with 10 rabbits in each group. The animal model of temporomandibular osteoarthropathy was established with the method of Changjia et al. The intervention group was treated with He-Ne laser irradiation on the buccal motor of the affected side for 5 min / d at Shangguan, Xiguan, Xiaguan, Aigong and Yifeng (5 min / d / d) for 10 days, while the sham model group was treated only with joint capsule incision and suture tissue. The temporomandibular joint (TMJ) was removed at different time points and the expression of Col-2P PRG-4 was detected by Western Blot before and after the intervention by HE staining. Results: in HE staining of degenerative condylar cartilage, the fibers were degraded, the cells in the proliferative layer and the transitional layer were arranged in disorder, the nucleus was crumpled, the nucleus was stained intensively, the fibrous layer of condylar cartilage after He-Ne laser treatment was slightly loosened, and some fibers were synthesized. The expression of Col-2P PRG-4 protein was not significantly different between normal group and sham model group at different time points, but the expression of extracellular matrix protein in model group was significantly decreased on the 11th day. After 10 days of He-Ne laser intervention, the expression of extracellular matrix protein increased significantly. Conclusion: He-Ne laser intervention can upregulate the expression of extracellular matrix (Col-2) PRG-4 in chondrocytes of temporomandibular joint, correct the degradation of extracellular matrix, and prevent and treat temporomandibular joint osteoarthritis.
【作者单位】：武汉市第一医院口腔科;
【基金】：武汉市卫生科研项目(WX14C03)
【分类号】：R782.6

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