双酸酸蚀钛表面复合含钙纳米薄片膜层对成骨细胞行为的影响

发布时间：2018-05-12 04:50

本文选题：钛 + 酸蚀　；参考：《南京医科大学学报(自然科学版)》2017年10期

【摘要】：目的:制备含钙纳米薄片膜层修饰的双酸酸蚀钛表面并评价其对成骨细胞行为的影响。方法:通过双酸酸蚀和氢氧化钙/双氧水混合溶液水热处理,制备2种含钙纳米薄片膜层修饰的双酸酸蚀钛表面(1 h、6 h处理组)。以大颗粒喷砂酸蚀(SLA)钛表面为对照组,2种含钙纳米薄片膜层修饰的双酸酸蚀钛表面为实验组,观察分析不同钛表面的微形貌和表面元素组成;将MC3T3-E1成骨细胞接种于各组试件表面,研究不同钛表面对成骨细胞生物学行为的影响。结果 :在双酸酸蚀钛表面制备形成2种形貌均一的纳米薄片膜层结构,均含有微量钙元素。相比于SLA钛表面,2种新型钛表面均有利于MC3T3-E1成骨细胞的黏附和增殖,显著增强成骨细胞的碱性磷酸酶活性,并上调成骨相关基因的表达。其中,1 h处理组性能更优。结论:双酸酸蚀钛表面复合含钙纳米薄片膜层能有效促进成骨细胞的黏附、增殖和分化。
[Abstract]:Aim: to prepare the surface of titanium bicarbonate modified by nano-slice film containing calcium and evaluate its effect on the behavior of osteoblasts. Methods: two kinds of calcium containing nanoscale films were prepared by hydrothermal treatment of calcium hydroxide / hydrogen peroxide mixed solution. The surface of titanium was treated for 1 h or 6 h. The micromorphology and surface element composition of different titanium surfaces were observed and analyzed on the surface of large particle sand-blast acid etching (SLAs) as control group and two kinds of thin film modified with calcium nanocrystalline film as experimental group, and the micromorphology and surface element composition of different titanium surfaces were observed and analyzed. The effects of different titanium surfaces on the biological behavior of osteoblasts were studied by inoculating MC3T3-E1 osteoblasts on the surface of each group of specimens. Results: two kinds of homogeneous nanocrystalline films were prepared on the surface of titanium bicarbonate, both of which contained trace calcium. Compared with the two new titanium surfaces on the surface of SLA, the two new titanium surfaces were beneficial to the adhesion and proliferation of MC3T3-E1 osteoblasts, significantly increased the alkaline phosphatase activity of osteoblasts and up-regulated the expression of osteoblast-associated genes. The performance of 1 h treatment group was better. Conclusion: titanium bicarbonate can effectively promote the adhesion, proliferation and differentiation of osteoblasts.
【作者单位】：南京医科大学口腔疾病研究江苏省重点实验室南京医科大学附属口腔医院种植科;芜湖市口腔医院综合科;上海市口腔医学重点实验室;
【基金】：国家自然科学基金面上项目(81472928) 江苏省卫生计生委面上科研课题(H201641) 江苏省青年医学人才项目(QNRC2016850) 江苏省“六大人才高峰”资助项目(2014-WSW-035) 东南大学-南京医科大学合作基金(2242017K3DN14) 江苏高校优势学科建设工程资助项目(2014-37)
【分类号】：R783.6

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