离体牙储存方式及玷污层对根尖微渗漏影响的实验研究

发布时间：2018-05-13 12:11

本文选题：根尖倒充填术 + 储存方法　；参考：《华北理工大学》2015年硕士论文

【摘要】：目的1比较不同的离体牙储存方法及储存时间对根尖微渗漏的影响,以期得出与新鲜离体牙实验结果相近的储存方法。2比较使用不同的根尖倒充填材料时,根管壁玷污层去除与否对根尖微渗漏的影响。方法1将收集的55颗新鲜单根管离体牙随机分入:1)实验组:采用4种不同的储存方式(每组各10颗)分别是4℃蒸馏水、4℃10%甲醛溶液、4℃2%氯亚明溶液和-20℃冷冻储存;2)对照组(5颗):新鲜离体牙(30分钟内用于实验);3)阳性对照与阴性对照组(每组各5颗)。在储存的第30天和第60天,随机从实验组抽取5个样本用于实验。所有样本常规根管预备后,冷牙胶侧向加压法充填根管,根尖切除后进行根管倒预备,实验组与对照组用GIC进行倒充填,去除根管上段充填物。所有样本在牙根表面(除牙根横断面)均匀涂布2层透明指甲油,恒温箱干燥。将样本置于检测微渗漏模型中。于第1、2、4、7、10、15、20天和30天检测从冠方向根方漏出的葡萄糖的浓度。2对所收集的130颗单根管牙进行常规根管预备后,冷牙胶侧向加压法充填根管,根尖切除后进行根管倒预备,将样本随机分为实验组和对照组(每组各65颗)。实验组:用17%EDTA+1%氯亚明荡洗根管;对照组:用5m L生理盐水荡洗根管。从实验组和对照组中各随机抽取5颗牙做扫描电镜观察。各组余下60颗分3组分别用MTA、GIC和银汞合金进行倒充填,将根管内上段充填物去除。所有样本在牙根表面(除牙根横断面)均匀涂布2层透明指甲油,恒温箱干燥。所有样本置于微渗漏模型中,于第1、2、4、7、10、15、20天和30天检测从冠方向根方漏出的葡萄糖的浓度。结果1阳性对照组在第1天可见乳胶管内液体显著下降;阴性对照组在观察期间未检测到葡萄糖的渗出。在实验组和对照组中各时间点上均能检测到不同程度的渗漏值。储存30天与储存60天测得的微渗漏值相比无显著差异(P0.05)。所有实验组中,-20℃冷冻储存方法的渗漏值最小,与其他实验组相比存在统计学差异(P0.05)。-20℃冷冻储存与对照组(新鲜牙)相比,渗漏值变化不大,无统计学差异(P0.05)。2实验组与对照组在各个时间点均能检测到葡萄糖。在离体牙去除或者保留玷污层中,MTA与GIC、银汞合金相比,MTA的渗漏值最小,而银汞合金的渗漏值最大,其差异具有统计学意义(P0.05)。MTA和GIC进行倒充填时,去除根管壁玷污层较保留玷污层的渗透值小,此差异具有统计学意义(P0.05);而银汞合金做倒充填时,去除与保留玷污层的渗透值相比无显著差异(P0.05)。结论1不同储存时间的离体牙用于根尖微渗漏实验,对根尖微渗漏实验不存在影响。在根尖微渗漏实验中,-20℃冷冻储存方法与新鲜离体牙所得实验结果相近。2MTA用于根尖倒充填所引起的根尖微渗漏明显低于GIC、银汞合金。应用MTA、GIC做根尖倒充填时,去除根管壁玷污层较之保留对其根尖封闭性更好,而用银汞合金做倒充填时玷污层去除与否对提高根尖封闭性无显著意义。
[Abstract]:Objective 1 to compare the effect of different storage methods and storage time on the microleakage of the root apex, in order to obtain the effect of the storage method,.2, which is close to the results of the fresh tooth test, to compare the effect of the root canal wall smear layer on the root tip microleakage when using different apex filling materials. Method 1 55 fresh single root canals were collected. Body teeth were randomly divided into 1) experimental group: 4 different storage methods (10 of each group) were 4 degrees of distilled water, 4 centigrade 10% Formaldehyde Solution, 4 C 2% chloramine solution and -20 centigrade freezing storage; 2) the control group (5): fresh isolated teeth (in 30 minutes for experiment), 3) positive control and negative control group (each of each 5). 5 samples were selected randomly from the experimental group for the experiment. After the routine root canal preparation, the root canals were filled with cold tooth glue, and the root canal was prepared after the root apex resection. The experimental group and the control group filled the root canal with GIC to remove the upper part of the root canal. All samples were evenly coated on the root surface (except the root cross section) with 2 layers of transparency. The samples were placed in the microleakage model. At the 1,2,4,7,10,15,20 and 30 days, the concentration of glucose leaked from the root square of the crown was detected by.2. The root canals were filled with the cold tooth gum lateral pressure method and the root canal was prepared after the apex resection, and the samples were followed. The experimental group was divided into the experimental group and the control group (65 in each group). The experimental group was used to wash the root canal with 17%EDTA+1% chloramine, and the control group was washed with 5m L saline. The 5 teeth were randomly selected from the experimental group and the control group for scanning electron microscopy. The remaining 60 3 groups were filled with MTA, GIC and amalgam, and the upper part of the root canal was filled. All samples were evenly coated on the root surface (except the root cross section) with 2 layers of transparent nail oil and the constant temperature box was dry. All samples were placed in the microleakage model, and the concentration of glucose leaked from the root square was detected at day 1,2,4,7,10,15,20 and 30 days. The results of 1 positive pairs showed a significant drop in the emulsion in first days. The negative control group did not detect the exudation of glucose during the observation period. The leakage values of different degrees were detected at all time points in the experimental group and the control group. There was no significant difference between the 30 days storage and the 60 day storage microleakage (P0.05). In all experimental groups, the leakage value of the freezing storage method at -20 centigrade was the smallest, with the other experimental groups. Compared with the control group (fresh teeth), the leakage value changed little compared with the control group (P0.05). There was no statistical difference between the control group and the control group (P0.05). The.2 experiment group and the control group were able to detect the glucose at all time points. In the removal or retention of the stained layer, the MTA and GIC, the silver amalgam, the minimum leakage value of MTA, and the silver mercury. The leakage value of the alloy is the largest, and the difference has statistical significance (P0.05).MTA and GIC for backfilling, the removal of the smear layer of the root canal wall is smaller than that of the retaining layer. The difference has statistical significance (P0.05), but when the silver amalgam is filled, there is no significant difference (P0.05) between the removal and the retention of the smear layer (P0.05). Conclusion 1 different reservoirs are stored. In the experiment of root tip microleakage, the -20 centigrade cryopreservation method was similar to that of fresh fresh teeth, and the apex microleakage caused by.2MTA for root apex filling was significantly lower than that of GIC, and silver amalgam was used to fill the root tip with MTA and GIC. The removal of the smear layer of the root canal wall is better than that of the retention of the root canal, but the removal of the tarnished layer is not significant to the enhancement of the apex sealing ability when the amalgam is used for backfilling.

【学位授予单位】：华北理工大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R781.05

【共引文献】