出生后小鼠牙不同发育期DKK1表达的形态学特点及其意义

发布时间：2018-05-13 14:12

本文选题：DKK + 牙发育　；参考：《吉林大学学报(医学版)》2017年02期

【摘要】：目的:探讨Wnt通路抑制因子Dickkopf1(DKK1)在出生后小鼠牙发育各阶段的时空表达特点,为阐明Wnt信号通路调控牙发育机制提供理论依据。方法:选择出生后第0.5、6.5、12.5、18.5、24.5和30.5天的昆明小鼠并按时间分组,每组3只。颈椎脱臼法处死小鼠,分离包含第一磨牙的下颌骨组织后进行固定、脱钙处理,制作厚度为5μm的下颌第一磨牙牙体-牙周组织联合切片。采用苏木素-伊红(HE)染色观察下颌第一磨牙的发育状态,应用免疫组织化学染色方法观察小鼠牙体组织和牙周组织中DKK1的表达特点。结果:小鼠出生后第0.5天时,下颌第一磨牙牙胚处于钟状晚期;第6.5天时,下颌第一磨牙牙釉质发育基本完成,可见上皮根鞘向根方延伸;第12.5天时,冠部牙本质发育完成,牙根形成约1/3;第18.5天时,牙根形成约2/3;第24.5天时牙根发育达到全长;第30.5天时根尖孔缩窄,牙根发育基本完成。小鼠出生后第0.5天时,牙胚中无DKK1阳性表达;第6.5天时,成牙本质细胞中DKK1呈弱表达;从第12.5天到30.5天,成牙本质细胞中DKK1仍呈阳性表达,而牙周膜中开始出现DKK1表达,且随时间延长表达逐渐增强,牙槽骨和细胞性牙骨质中也出现DKK1的阳性表达,牙髓组织中始终呈阴性表达。结论:出生后小鼠牙不同发育期DKK1呈规律性表达,提示其可能参与了牙本质和牙周组织的发育过程。
[Abstract]:Aim: to investigate the temporal and spatial expression of Wnt pathway inhibitor Dickkopf1 DKK1 in postnatal mouse tooth development, and to provide theoretical basis for elucidating the mechanism of tooth development regulated by Wnt signaling pathway. Methods: Kunming mice were divided into three groups according to their time. The mice were killed by cervical dislocations, the mandibular tissues containing the first molars were separated and fixed, decalcified, and the mandibular first molars with thickness of 5 渭 m were made into the joint section of the mandibular first molar and periodontal tissue. The developmental state of mandibular first molar was observed by hematoxylin-eosin-hehe staining and the expression of DKK1 in mouse tooth and periodontal tissue was observed by immunohistochemical staining. Results: on the 0.5 day after birth, the first mandibular molar tooth germ was in the late bell shape, at 6.5 days, the enamel development of the first mandibular molar was basically completed, and the epithelial root sheath extended to the root, and on the 12.5 day, the development of the dentin of the crown was completed. Root formation was about 1 / 3; at 18.5 days, root formation was about 2 / 3; at 24.5 days, the root development reached full length; at 30.5 day, the apical foramen narrowed and root development was basically completed. There was no positive expression of DKK1 in dental germ at 0.5 day after birth, weak expression of DKK1 in odontoblast at 6.5 day, DKK1 expression in odontoblast from 12.5 day to 30.5 day, and DKK1 expression in periodontal membrane. The positive expression of DKK1 was also found in alveolar bone and cellular cementum, but negative in pulp tissue. Conclusion: the expression of DKK1 in different developmental stages of postnatal mouse teeth is regular, which suggests that DKK1 may be involved in the development of dentin and periodontal tissue.
【作者单位】：吉林大学口腔医院正畸科;吉林省牙发育及颌骨重塑与再生重点实验室;
【基金】：国家自然科学基金资助课题(81470764) 吉林省卫计委青年科研基金资助课题(2015Q003) 吉林大学白求恩计划项目资助课题(2015321) 吉林省科技厅科技发展计划项目资助课题(20160520156JH)
【分类号】：R781

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