五倍子水提取物对人牙髓细胞Ⅰ型胶原合成的影响

发布时间：2018-05-16 03:03

  本文选题：五倍子水提取物 + 体外培养　； 参考：《牙体牙髓牙周病学杂志》2014年11期

【摘要】：目的:观察五倍子水提取物对人牙髓细胞Ⅰ型胶原合成的影响。方法:取第6代体外培养的人牙髓细胞分别与终末浓度为5、10μg/m L的五倍子水提取物共同培养3 d后,采用免疫组化染色法观察人牙髓细胞中Ⅰ型胶原的表达情况,并用HPIAS-1000图像分析系统进行定量分析。结果:对照组和不同浓度五倍子水提取物作用的实验组人牙髓细胞中均有Ⅰ型胶原的表达,其免疫反应物质多定位于胞浆。人牙髓细胞经5、10μg/m L浓度的五倍子水提取物作用后,其Ⅰ型胶原的表达水平均明显增高,其中以10μg/m L组的增高程度最大,各组间两两相比差异均有统计学意义(P0.05)。结论:5、10μg/m L五倍子水提取物均能明显促进人牙髓细胞中Ⅰ型胶原的表达;提示其具有促进牙髓细胞向成牙本质细胞分化的作用。
[Abstract]:Aim: to observe the effect of gallnut water extract on collagen type I synthesis in human dental pulp cells. Methods: human dental pulp cells cultured in vitro were cultured with 5 渭 g / mL gallnut water extract (5 渭 g / mL) for 3 days. The expression of type I collagen in human dental pulp cells was observed by immunohistochemical staining. Quantitative analysis was carried out with HPIAS-1000 image analysis system. Results: the expression of type I collagen was found in human dental pulp cells in the control group and in the experimental group treated with different concentrations of gallnut water extract. The immunoreactive substances were mostly localized in the cytoplasm. The expression of type 鈪，

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