牙胚外伤及氟牙症对大鼠牙釉质发育影响的实验研究

发布时间：2018-05-16 10:55

本文选题：牙胚外伤 + 氟斑牙　；参考：《郑州大学》2017年硕士论文

【摘要】：实验目的建立大鼠牙胚外伤及氟斑牙引起牙釉质发育异常模型,对发育异常牙进行冷光美白,酸蚀处理,观察其微观变化,为临床美学治疗提供参考。实验方法将1d龄30只SD大鼠随机分为三组,每组10只。空白对照组(A组)大鼠自由生长。牙胚外伤组(B组),在大鼠下颌切牙区,平行于下颌前牙生长方向,对大鼠下颌骨垂直加压一10N/5mm2的力,建立牙胚外伤大鼠动物模型。氟斑牙组(C组),待大鼠3周龄,配制50mg/l的氟化水,每天自由饮用,建立氟斑牙动物模型。实验1个月后,动物模型建立,记录牙齿发育状况。然后引颈处死大鼠,剥离大鼠下切牙。再将各组实验牙(每组18颗)随机分为3组(n=3),每组6颗牙齿,进行空白对照(分别标记为A0,B0,CO),冷光美白处理(分别标记为A1,B1,C1),酸蚀处理(分别标记为A2,B2,C2).然后将各组实验牙进行处理前后大体比较,电镜扫描观察及能谱分析,观察各组实验牙在不同处理前后的变化及釉质表面微观形态和矿物元素Ca,P含量的变化。结果1.牙胚外伤及高浓度氟都可引起牙釉质的发育异常。2.牙齿发育情况:A组牙齿均正常萌出,牙釉质发育未见异常。B组出现牙齿移位萌出4例,单个下前牙萌出2例,釉质出现白斑6例。C组出现釉质表面白垩斑4例,棕白相间条纹6例,牙釉质缺损4例。3.牙釉质比较:正常牙釉质表面光滑,牙呈淡黄色,颜色均匀。牙胚外伤牙釉质表面有白斑形成,与正常釉质界限清楚,在无白斑处,牙釉质表现为乳白色。氟斑牙釉质大面积白垩斑,或有黄白相间条纹,牙齿黄染,有牙釉质缺损。冷光美白后肉眼观察三组牙变化均不明显。在酸蚀后三组牙釉质表面均变成白色不透明,氟斑牙黄染去除。4.SEM结果示:表面未处理时,正常牙A0组牙齿表面平整光滑,牙釉质表面有一层无结构釉质覆盖,无牙釉质缺损。外伤牙B0组,牙齿表面整体光滑,出现较多裂隙,或可见釉质表面凸凹不平,出现釉质脱矿区。氟斑牙C0组非白垩斑区牙釉质表面光滑,与正常牙无区别,白垩斑区釉质表面粗糙,在釉质缺损区可见暴露的釉柱结构及大面积裂纹存在,部分牙釉质缺损可见深层牙本质结构。冷光美白处理后:A2组可见牙齿表面变化不明显,仅在高倍镜下可见表面变得粗糙,出现一些小的凸凹不平。B2组矿化不良区釉质表面脱矿明显。C2组可见釉质表面脱矿不明显,或仅有鳞屑状脱矿。酸蚀处理后:A1组能清晰看到釉柱结构清晰,排列整齐。釉柱与釉间隙界限分明。B1组牙釉质结构可见,与釉间隙界限清楚,但釉柱间距增宽,排列无正常牙整齐。C1组可见釉柱部分缺失,晶体结构松散,釉柱排列不紧密。5.能谱分析结果示:未处理牙釉质表面,在外伤牙,氟斑牙釉质发育异常区Ca,P含量较正常牙低(P0.05)。冷光美白后B1,C1与A1相比,Ca,P含量均有所增高(P0.05),但B1,C1间比较无统计学意义。在酸蚀后,A2,B2,C2三组间Ca,P含量比较无统计学意义(P0.05)。结论牙胚外伤引起的牙釉质发育异常与氟斑牙引起的牙釉质发育异常表面结构存在一定的差异,其表面结构对冷光美白的敏感性存在差异,在临床工作中针对不同原因引起的牙釉质发育不全的处理要不同。
[Abstract]:Objective to establish an abnormal model of tooth enamel development caused by dental trauma and dental fluorosis in rats. Cold light whitening, acid etching treatment and microscopic changes were used to provide reference for clinical aesthetic treatment. 30 SD rats of 1D age were randomly divided into three groups, 10 rats in each group. The free growth of the blank control group (group A). The injury group (group B), in the mandibular incisor region, parallel to the direction of the mandibular anterior tooth growth, the rat model of tooth embryo trauma was established by the force of one 10N/5mm2 in the vertical compression of the mandible of the rat. The dental fluorosis group (group C) was 3 weeks old, and the fluorinated water of 50mg/l was prepared, and the model of dental fluorosis was established every day. The animal model after 1 months of experiment was the animal model. And then the tooth development was recorded. Then the rats were killed and the rats were stripped to the lower incisors. Then the experimental teeth (18 in each group) were randomly divided into 3 groups (n=3), each group had 6 teeth, and the blank control (labeled as A0, B0, CO), the cold light whitening treatment (respectively labeled as A1, B1, C1), acid etching treatment (respectively marked as A2, B2, C2). Then each experiment was carried out. General comparison of teeth before and after treatment, scanning electron microscope observation and energy spectrum analysis, observed the changes of the teeth before and after different treatments, the micromorphology of the enamel surface and the change of the mineral element Ca, P content. Results 1. tooth embryo trauma and high concentration of fluoride can cause the development of the tooth enamel with abnormal.2. tooth development: the teeth of group A are normally erupted. There were 4 cases of tooth displacement and eruption in group.B, 2 cases of single anterior teeth eruption and 6 cases of enamel surface Cretaceous spot in group.C, 6 cases of brown and white stripes, 4 cases of enamel defect and 4.3. enamel, the normal enamel surface was smooth, the tooth was pale yellow, and the enamel surface of tooth embryo trauma was white. The plaque was formed and clearly defined with the normal enamel. In the absence of leukoplakia, the enamel showed milk white. The large area of Cretaceous plaque in the enamel of the dental fluorosis, or the yellow and white stripes, the teeth yellow dye, and the enamel defect. After the cold light whitening, the changes of the three groups of teeth were not obvious. The three groups of enamel surface became white and opacity after acid etching, and the dental fluorosis was yellow. The results of dyeing and removal of.4.SEM showed that when the surface was not treated, the surface of the normal tooth A0 was smooth and smooth, and the enamel surface had a layer of unenamel covering and no enamel defect. In group B0 of the traumatic teeth, the surface of the teeth was smooth, more fissures appeared, or the enamel surface was not flat, and the enamel demealization appeared. The enamel enamel in group C0 of dental fluorosis was not Cretaceous area. The quality of the surface was smooth, and there was no difference from the normal teeth. The enamel surface was rough in the Cretaceous area. The exposed enamel structure and large area cracks were found in the enamel defect area, and the deep dentin structure was visible in some enamel defects. After cold light whitening treatment, the A2 group showed that the surface changes of the teeth were not obvious and the surface became rough and appeared only under high magnification. The enamel surface demineralization of some small convex and concave.B2 group mineralized areas was obvious in group.C2. The demineralization of enamel surface was not obvious, or only scaly demineralization. After acid etching treatment: the A1 group could clearly see the structure of the glaze column clear and orderly. The enamel structure of the.B1 group was clearly visible, but the boundary between the glaze and the glaze was clear, but the glaze column was clear, but between the glaze columns. In.C1 group without normal teeth, partial deletion of the enamel column, loose crystal structure and non tight.5. spectrum analysis showed that the enamel surface was not treated, Ca in the traumatic tooth, Ca of enamel development in the dental fluorosis, P content was lower than that of normal tooth (P0.05). B1, C1 and A1, C1 and A1, C1 and P content increased (P0.05). There was no statistical significance between B1 and C1. After acid etching, there was no significant difference between Ca and P in A2, B2 and C2 three groups (P0.05). Conclusion the abnormal enamel development caused by tooth germ injury and abnormal surface structure of enamel induced by dental fluorosis were different, and the sensitivity of surface structure to cold light whitening was different in clinical work. The treatment of enamel hypoplasia caused by different reasons is different.

【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R783

【参考文献】