雷尼酸锶、强骨胶囊对骨质疏松大鼠正畸牙移动药物作用的研究

发布时间：2018-05-17 12:51

本文选题：骨质疏松 + 牙移动　；参考：《华北理工大学》2017年硕士论文

【摘要】：目的通过建立大鼠骨质疏松、正畸牙移动实验动物模型,同时给予雷尼酸锶和强骨胶囊药物干预,检测正畸加力后大鼠不同阶段的胫骨近端骨密度、上颌第一磨牙移动距离以及牙周组织中胰岛素样生长因子1(IGF-1)和转化生长因子1(TGF-β1)的表达情况。探讨药物干预后对骨质疏松模型大鼠正畸牙移动的影响以及不同药物的治疗效果。为临床骨质疏松患者正畸治疗选择干预药物提供实验依据。方法实验选取80只4周龄SD雄性大鼠,体重为(210±10)g,置于SPF级环境中饲养,大鼠自由饮食水。80只大鼠随机分成实验对照组(A组),模型对照组(B组),雷尼酸锶组(C组),强骨胶囊组(D组),每组各20只。实验性喂养一周后B、C、D组建立骨质疏松模型,给予维甲酸80mg/(kg·d)连续灌胃2周,A组给予等量蒸馏水作为对比。两周后每组随机处死5只大鼠行骨密度检测(bone mass density,BMD),确定建模成功。建模成功后,给予D组强骨胶囊溶剂90mg/(kg·d);给予C组雷尼酸锶溶剂400mg/(kg·d),进行灌胃给药;A组、B组则给予等量蒸馏水进行对比。在用药物干预治疗两周以后,所有大鼠安装正畸装置,加力后第7d、14d、21 d分批处死,每次随机处死5只。取大鼠上颌第一磨牙及附近牙槽骨,测量加力侧大鼠第一磨牙牙移动距离并进行HE染色和免疫组化染色,应用半定量法分析其牙周组织中IGF-1和TGF-β1的表达情况。结果1 B、C、D组大鼠胫骨BMD值均低于A组大鼠,差异有统计学意义(P0.05)。2同一加力时间段内B组大鼠的第一磨牙移动距离高于D、C、A组大鼠,差异有统计学意义(P0.05)。3 C组和D组大鼠牙移动速度较B组有所减慢,差异有统计学意义(P0.05)。4免疫组织化学的检测结果显示,同一时间点C组和D组IGF-1表达较B组显著增加,且7d和14d时表达较显著,21天时表达有所降低,差异有统计学意义(P0.05);同一时间点C组IGF-1表达显著强于D组,差异有统计学意义(P0.05)。5免疫组织化学的检测结果还显示,同一时间点C组和D组TGF-β1表达较B组显著增加,且7d和14d时表达较显著,21天时表达有所降低,差异有统计学意义(P0.05);同一时间点C组TGF-β1表达显著强于D组,差异有统计学意义(P0.05)。结论1实验证实SD雄性大鼠给予维甲酸80mg/(kg·d)连续灌胃2周,可造成骨质疏松模型。2骨质疏松会使正畸大鼠的牙移动速度加快。3雷尼酸锶与强骨胶囊均能降低骨质疏松并使牙周组织中IGF-1和TGF-β1高表达,提示成骨作用增强。4雷尼酸锶对骨质疏松药物作用强于强骨胶囊。
[Abstract]:Objective to establish an experimental model of osteoporosis and orthodontic tooth movement in rats, and to measure the proximal tibia bone density of rats at different stages after orthodontic treatment with strontium ranidate and Qianggu capsule. The movement distance of maxillary first molar and the expression of insulin-like growth factor (IGF-1) and transforming growth factor (TGF- 尾 _ 1) in periodontal tissues. To investigate the effect of drug intervention on orthodontic tooth movement in osteoporosis model rats and the therapeutic effects of different drugs. To provide experimental evidence for the choice of intervention drugs for orthodontic treatment in patients with osteoporosis. Methods Eighty 4-week-old SD male rats, weighing 210 卤10g, were fed in SPF environment. The rats were randomly divided into three groups: experimental control group (n = 20), model control group (n = 20), strontium raniate group (n = 20), group C (n = 20) and group D (n = 20). Osteoporosis model was established in group D after experimental feeding for one week. The rats in group A were given the same amount of distilled water for 2 weeks after continuous administration of retinoic acid (80mg/(kg d). Two weeks later, 5 rats in each group were randomly killed to measure bone mass density. After modeling successfully, group D was given 90mg/(kg dai, group C was treated with strontium ranionate, and group A was given the same amount of distilled water. After two weeks of drug intervention, all rats were installed with orthodontic devices, and were killed in batches on day 7, 14 and day 21 respectively, and 5 rats were randomly killed each time. The maxillary first molar and its adjacent alveolar bone were taken to measure the distance of tooth movement, HE staining and immunohistochemical staining. The expression of IGF-1 and TGF- 尾 1 in periodontal tissue was analyzed by semi-quantitative method. Results (1) the tibia BMD values of rats in group B were lower than those in group A, and the difference was statistically significant. The distance of first molar movement in group B was higher than that in group A during the same period of exertion. The rate of tooth movement in group C and group D was slower than that in group B, and the expression of IGF-1 in group C and group D was significantly higher than that in group B at the same time point, and the difference was statistically significant (P 0.054.The results of immunohistochemistry showed that the expression of IGF-1 in group C and group D was significantly higher than that in group B at the same time point). The expression of IGF-1 in group C was significantly higher than that in group D at day 7 and day 14, and the expression of IGF-1 in group C was significantly higher than that in group D at the same time point, and the results of immunohistochemistry showed that the expression of IGF-1 in group C was significantly higher than that in group D at the same time point. At the same time point, the expression of TGF- 尾 1 in group C and group D was significantly higher than that in group B, and the expression of TGF- 尾 1 in group C was significantly lower than that in group B at day 7 and day 14, the difference was statistically significant (P 0.05), and the expression of TGF- 尾 1 in group C was significantly higher than that in group D at the same time point (P 0.05). Conclusion 1Sprague-Dawley male rats were given retinoic acid (80mg/(kg d) for 2 weeks. 2 osteoporosis could accelerate tooth movement in orthodontic rats. 3. 3 strontium raniate and Qianggu capsule could decrease osteoporosis and increase the expression of IGF-1 and TGF- 尾 1 in periodontal tissue. The results suggest that the osteogenic effect is stronger than that of Qianggu capsule.
【学位授予单位】：华北理工大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R783.5

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