唑来膦酸对牙龈成纤维细胞生物学特性的影响研究

发布时间：2018-05-18 13:48

本文选题：唑来膦酸 + 牙龈成纤维细胞　；参考：《口腔医学研究》2017年11期

【摘要】：目的:探讨唑来膦酸对牙龈成纤维细胞增殖、凋亡、分化的影响及机制。方法:从牙龈组织分离原代人牙龈成纤维细胞(HGFs),0.5、1、5、10μmol/L的唑来膦酸处理HGFs 24、48、72h后,CCK8实验检测细胞的增殖;流式细胞仪检测10μmol/L的唑来膦酸处理HGFs 72h后的细胞凋亡情况;加入10μmol/L的唑来膦酸的成骨诱导分化液培养HGFs 7d后,碱性磷酸酶(ALP)试剂盒检测ALP活性,Western blot检测Runx2和OPN的蛋白表达;10μmol/L的唑来膦酸处理HGFs 72h后,Western blot检测NF-κB信号通路p65、IκBα蛋白表达。结果:唑来膦酸可呈剂量依赖抑制HGFs细胞增殖(P0.01);唑来膦酸组细胞的凋亡率显著高于对照组,ALP活性及Runx2、OPN蛋白表达显著高于对照组,p65、IκBα的蛋白表达显著低于对照组(P0.01)。结论:唑来膦酸可抑制HGFs增殖,促进细胞的凋亡和分化,其机制与下调NF-κB信号通路有关。
[Abstract]:Aim: to investigate the effect and mechanism of zoledronic acid on the proliferation, apoptosis and differentiation of gingival fibroblasts. Methods: the primary human gingival fibroblasts were isolated from gingival tissue and treated with 10 渭 mol/L of zoledronic acid for 72 hours. The proliferation of cells was detected by flow cytometry after treated with 10 渭 mol/L of zoledronic acid for 72 hours, and the apoptosis of cells treated with 10 渭 mol/L of zoledronic acid for 72 hours was detected by flow cytometry. After HGFs was cultured with 10 渭 mol/L of zoledronic acid for 7 days, the activity of ALP was detected by alkaline phosphatase assay kit. The protein expression of Runx2 and OPN was detected by Western blot. The protein expression of Runx2 and OPN was detected after treatment with 10 渭 mol/L zoledronic acid for 72 hours. Western blot was used to detect the expression of p65 I 魏 B 伪 protein in NF- 魏 B signaling pathway. Results: Zoledronic acid inhibited the proliferation of HGFs cells in a dose-dependent manner, and the apoptotic rate of Zoledronic acid group was significantly higher than that of control group (P 0.01). Conclusion: Zoledronic acid can inhibit the proliferation of HGFs and promote cell apoptosis and differentiation, and its mechanism is related to down-regulation of NF- 魏 B signaling pathway.
【作者单位】：牡丹江医学院第二附属医院口腔科;牡丹江医学院第二附属医院五官科;
【基金】：黑龙江省自然科学基金项目(编号:H201596)
【分类号】：R781.42

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