不同染液对Nd:YAG激光封闭牙本质小管效果的影响

发布时间：2018-05-18 22:26

本文选题：Nd + YAG激光　；参考：《牙体牙髓牙周病学杂志》2014年10期

【摘要】：目的:观察不同染液对Nd:YAG激光封闭牙本质小管效果的影响。方法:取4个新鲜拔除的第一恒磨牙,用低速金刚砂盘水平去除冠部牙釉质并暴露牙本质后,在每个牙本质样本的表面各划分出4个面积为2 mm×3 mm的操作区(共16个区域);分别用EDTA进行表面处理后,将16个区随机分配到4个组中(n=4),其中不涂染液直接激光照射的样本为对照,其他3组样本分别涂20 g/L甲基紫、墨汁、20 g/L亚甲蓝后,用Nd:YAG激光以120 mJ、5 Hz(对应的激光能量密度为200 J/cm2)扫描式照射各操作区20 s;然后常规制备SEM标本,在SEM下观察,同时采集1500×和3000×视野下的典型图像,分别用于计算各组牙本质小管的封闭率和测量小管口的直径。结果:对照组、20 g/L甲基紫组、墨汁组、20 g/L亚甲蓝组的牙本质小管封闭率分别为(10.01±1.71)%、(2.72±0.70)%、(11.18±2.34)%、(36.14±6.30)%,各组间两两相比P0.05;小管口直径(μm)分别为2.75±0.26、3.25±0.24、1.61±0.13、1.32±0.14,各组间两两相比P0.05。结论:20 g/L亚甲蓝染液能明显增强Nd:YAG激光对牙本质小管的封闭作用。
[Abstract]:Objective: to observe the effect of different dyes on the effect of Nd:YAG laser sealing of dentine tubules. Methods: four freshly extracted first permanent molars were removed and the enamel of the crowns was removed horizontally with a low speed Emery plate after the dentin was exposed. The surface of each dentin sample was divided into four operating areas (2 mm 脳 3 mm), which were treated with EDTA. The 16 regions were randomly assigned to 4 groups. The samples without direct laser irradiation were compared. The other three groups were treated with 20 g / L methylviolet and 20 g / L methylene blue respectively. The operating regions were irradiated with Nd:YAG laser at 120mJ / cm ~ 5 Hz (corresponding energy density of 200J / cm ~ 2) for 20 s, then the SEM specimens were routinely prepared and observed under SEM, and the typical images of 1500 脳 and 3000 脳 field of vision were collected at the same time. The closure rate of dentin tubules and the diameter of tubule orifice were calculated. Results: the closure rate of dentine tubules in 20 g / L methyl violet group and 20 g / L methylene blue group were 10.01 卤1.71 卤2.72 卤0.70 and 11.18 卤2.34 卤36.14 卤6.30, respectively, and the diameter of tubule orifice (渭 m) was 2.75 卤0.261.61 卤0.131.61 卤0.131.32 卤0.14, respectively. Conclusion 20 g / L methylene blue dye can significantly enhance the blocking effect of Nd:YAG laser on dentine tubules.
【作者单位】：南京医科大学口腔医学研究所;南京医科大学附属口腔医院牙体牙髓病科;
【基金】：国家自然科学基金资助项目(81371144) 江苏省高校优势学科建设工程资助项目[PAPD(2011-137)]
【分类号】：R781.2

【参考文献】