纳米银对多菌种生物膜中粪肠球菌的杀菌作用

发布时间：2018-05-20 16:06

本文选题：纳米银 + 叠氮溴化丙锭　；参考：《实用口腔医学杂志》2017年03期

【摘要】：目的:研究纳米银对多菌种生物膜中粪肠球菌的杀菌作用。方法:用粪肠球菌、产黑色素普雷沃菌以及具核梭杆菌构建多菌种生物膜85个。分别用0.1%(12~15 nm、100 nm)纳米银悬浊液以及2%的次氯酸钠溶液处理,然后通过qPCR方法检测样本中粪肠球菌的活菌量和粪肠球菌的细菌总量。配对t检验比较各组细菌样本临界循环数(Ct)。结果:各个实验组使用和不使用PMA之间粪肠球菌的细菌计数有着差别(P=0.000);将3个实验组使用和不使用PMA q PCR检测到的Ct值的差值进行比较:纳米银(12~15 nm)实验组最大,纳米银(100 nm)实验组次之,次氯酸钠实验组最小(P0.05)。结论:纳米银对于多菌种生物膜中的粪肠球菌有着较强的杀菌作用,直径较小的纳米银杀菌作用较强。
[Abstract]:Objective: to study the bactericidal effect of silver nanoparticles on Enterococcus faecalis in multispecies biofilm. Methods: 85 multispecies biofilms were constructed by Enterococcus faecalis, Prevobacter melanin producing bacteria and Clostridium nucleatum. The suspensions of silver nanoparticles and 2% sodium hypochlorite solution were used to detect the live bacteria of Enterococcus faecalis and the total bacteria of Enterococcus faecalis by qPCR. The critical cycle number of bacterial samples in each group was compared by paired t test. Results: there were significant differences in bacterial count of Enterococcus faecalis between the three experimental groups using and without PMA, and the difference of Ct values detected between the three experimental groups using and without PMA q PCR was the highest. Nano silver (100 nm) was the second in the experimental group, and sodium chlorate in the experimental group was the smallest (P 0.05). Conclusion: silver nanoparticles have strong bactericidal effect on Enterococcus faecalis in multi-species biofilm, and silver nanoparticles with smaller diameter have stronger bactericidal effect.
【作者单位】：合肥市口腔医院牙体牙髓二科;
【基金】：合肥市自主创新政策“借转补”项目(合科[2014]71号)
【分类号】：R781.05

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