TLR2在大鼠根尖周炎中的表达研究

发布时间：2018-05-21 03:32

本文选题：根尖周炎 + TLR2　；参考：《大连医科大学》2014年硕士论文

【摘要】：目的:本文通过建立大鼠根尖周炎动物模型研究Toll样受体2在大鼠根尖周炎中的表达。为根尖周炎的发病机制及治疗提供一定的理论依据。方法:选取SD大鼠20只,建立大鼠根尖周炎动物模型,以大鼠右侧下颌第一磨牙开髓后,髓腔内封入PBS缓冲液,髓腔采用进口玻璃离子暂封。于术后1周、2周、3周、4周四个时间点随机各取5只鼠,进行固定后,剖取大鼠下颌骨组织,脱钙,制作冰冻切片。采用免疫组织化学染色法检测Toll样受体2在大鼠根尖周炎组织中的表达与分布。结果:1周、2周、3周、4周四个时间点中TLR2在根尖周炎症区域中均呈阳性表达。TLR2的表达呈倒V型,即TLR2的表达在前三周呈上升趋势,并于三周时达到顶峰,然后开始逐渐降低。TLR2阳性细胞高于正常对照组,差异具有统计学意义(P㩳0.05)。结论:TLR2在大鼠实验性根尖周炎模型中表达并且TLR2的表达情况与根尖周炎密切相关。
[Abstract]:Aim: to study the expression of Toll-like receptor 2 in periapical periodontitis in rats. To provide a theoretical basis for the pathogenesis and treatment of periapical periodontitis. Methods: 20 SD rats were selected to establish the rat model of periapical periodontitis. After the right mandibular first molar was opened, PBS buffer solution was inserted into the pulp cavity, and the imported glass ion was used to seal the pulp cavity. Five rats were randomly selected at four and four points at 1 week, 2 weeks, 3 weeks and 4 weeks after operation. After fixation, the mandibular tissues of the rats were cut off, decalcified, and frozen sections were made. Immunohistochemical staining was used to detect the expression and distribution of Toll like receptor 2 in rat periapical periodontitis. Results the positive expression of TLR2 in periapical inflammatory region was inverted V type, that is, the expression of TLR2 increased in the first three weeks, and reached its peak at 3 weeks. Then the positive cells of. TLR2 were gradually decreased compared with the normal control group, and the difference was statistically significant (P < 0. 05). Conclusion the expression of TLR2 in rat model of periapical periodontitis and the expression of TLR2 are closely related to periapical periodontitis.
【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R781.3

【相似文献】