吸烟个体血清在具核梭杆菌侵入KB细胞及分泌MMP-1中的作用

发布时间：2018-06-02 09:52

本文选题：吸烟 + 具核梭杆菌　；参考：《实用口腔医学杂志》2014年06期

【摘要】：目的:探讨慢性牙周炎患者中吸烟个体血清在具核梭杆菌(Fn)侵入KB细胞及在侵入过程中对KB细胞分泌基质金属蛋白酶-1(MMP-1)的作用。方法:提取慢性牙周炎吸烟个体10例(Y组)、慢性牙周炎非吸烟个体10例(N组)、牙周健康非吸烟个体5例(H组)的血清。分别将各组血清200、400、800μl添加至Fn感染KB细胞模型,作用24 h后计数细菌菌落。用人酶联免疫吸附测定试剂盒检测各组上清液中MMP-1的浓度。用单因素方差分析进行数据分析。结果:3组800μl血清引起Fn侵入KB细胞的侵入率(%)分别为12.59±1.27、8.03±0.075和7.99±0.14(P0.05),KB细胞分泌MMP-1的浓度(μg/L)分别为400.04±21.02、252.57±18.89、262.47±35.29(P0.05)。200μl和400μl血清组与N组、H组比较,Fn侵入率及KB细胞MMP-1分泌量无统计学差异(P0.05)。结论:较高剂量吸烟个体血清有利于Fn侵入KB细胞并促进KB细胞分泌MMP-1。
[Abstract]:Objective: to investigate the effect of serum from smoking patients with chronic periodontitis on invasion of KB cells and secretion of matrix metalloproteinase-1 (MMP-1) in KB cells. Methods: the serum samples were extracted from 10 smokers with chronic periodontitis, 10 patients with chronic periodontitis and 5 healthy nonsmokers. Serum 200400800 渭 l was added to the KB cell model infected with FN for 24 h, and the bacterial colonies were counted. The concentration of MMP-1 in supernatant of each group was detected by Elisa kit. Single factor analysis of variance (ANOVA) was used to analyze the data. Results the invasion rate of FN invading KB cells was 12.59 卤1.270.03 卤0.075 and 7.99 卤0.14P0.05KB cells respectively (渭 g / L) in the three groups (渭 g / L) were 400.04 卤22252.57 卤18.89262.47 卤35.29(P0.05).200 渭 l and 400 渭 l, respectively. There was no significant difference in the invasion rate of FN and the MMP-1 secretion of KBP0.05 cells between the two groups. Conclusion: the higher dose of smoking serum is beneficial to the invasion of FN into KB cells and the secretion of MMP-1 by KB cells.
【作者单位】：中国医科大学口腔医学院牙周科;辽宁省口腔医学研究所牙周病研究室;
【基金】：国家自然科学基金(编号:81271153) 中国医科大学口腔医学院青年科研启动基金(编号:K101593-12-36)
【分类号】：R781.4

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