Zyflamend对实验性口腔癌抑制作用的研究

发布时间：2018-06-02 14:41

本文选题：Zyflamend + 口腔癌　；参考：《实用口腔医学杂志》2014年02期

【摘要】：目的:观察抗炎中药混合制剂Zyflamend(ZF)对DMBA诱发的金黄地鼠口腔癌的抑制作用。方法:116只金黄地鼠,10只设为阴性对照,其余用0.5%的DMBA石蜡油涂于地鼠左侧颊囊,3次/周,共涂6周。从第7周开始,将动物分为3组。给低浓度组(n=33)地鼠左侧颊囊局部涂抹1∶1石蜡油稀释的ZF,高浓度组(n=33)涂ZF原液,3次/周,连续18周,阳性对照组(n=40)不给药。第24周末处死动物,处死前2 h腹腔注射50 mg/kg的5-溴脱氧尿嘧啶(BrdU)。取左侧颊囊进行组织病理学观察和BrdU免疫组化染色。结果:未防治组低、高浓度ZF组,肉眼观察肿瘤发生率分别为86.7%、53.6%(P0.01)和50.0%(P0.01);组织病理学观察鳞状细胞癌的发病率分别为86.7%、78.6%(P0.05)和64.3%(P0.05)。ZF高浓度组的病变中组织,BrdU的增殖指数显著降低(P0.05)。结论:Zyflamend能够降低DMBA诱导金黄地鼠口腔癌的发生率,其机制可能与抑制细胞增殖有关。
[Abstract]:Aim: to observe the inhibitory effect of Zyflamendment ZF on oral cancer induced by DMBA in hamsters. Methods 10 golden hamsters were used as negative control, and the rest were coated with 0.5% DMBA paraffin oil on the left cheek pouch of hamster three times a week for 6 weeks. From week 7, animals were divided into 3 groups. The left cheek pouch of the hamster was locally smeared with 1:1 paraffin oil diluted ZF, and the high concentration group was treated with ZF solution 3 times a week for 18 weeks. Animals were killed at the end of the 24th week, and 50 mg/kg 5-bromodeoxyuridine (BrdU) was injected intraperitoneally 2 hours before death. The left cheek pouch was taken for histopathological observation and BrdU immunohistochemical staining. Results: the incidences of tumor were 86.7% (P 0.01) and 50.01% (P 0.01), respectively, and the incidences of histopathological observation were 86.7% (P 0.05) and 78.6% (P 0.05), respectively, and the proliferative index of BrdU was significantly decreased in the group of 64.3%(P0.05).ZF with high concentration of ZF (86.7%) and the group of 64.3%(P0.05).ZF with high concentration (P 0.01), and the incidence of histopathological observation of squamous cell carcinoma was 86.7% (P 0.05) and the proliferative index of BrdU was significantly lower than that of 64.3%(P0.05).ZF group (P 0.05). ConclusionZyflamend can reduce the incidence of oral cancer induced by DMBA in golden hamsters, and its mechanism may be related to the inhibition of cell proliferation.
【作者单位】：大连大学附属中山医院;首都医科大学口腔医学院;
【基金】：辽宁省教育厅项目(编号:L2012438)
【分类号】：R739.8

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