4种根管封闭剂体外细胞毒性的比较

发布时间：2018-06-04 10:26

本文选题：根管封闭剂 + CCK-法　；参考：《牙体牙髓牙周病学杂志》2014年02期

【摘要】：目的:评价4种根管封闭剂的生物相容性。方法:本实验通过X射线能谱仪对MTA、AH Plus、Roeko Seal、Cortisomol 4种根管封闭剂进行元素分析,并采用CCK-8法检测各材料凝固后即刻和1周时在100%和50%两种浸提液浓度下对L-929细胞的体外细胞毒性作用。结果:同一固化时间组内两种浸提液浓度相比,AH Plus组无论是固化即刻组还是固化后1周组,当其浸提液浓度为100%时,1、2、4 d各时间点的细胞增殖率均低于50%浸提液(P0.05),而其他3种封闭剂的即刻组和1周组样本中100%与50%浓度浸提液相比,各时间点的细胞增殖率均无显著性差异(P0.05)。同一时间点内各封闭剂相比,无论是固化即刻还是固化后1周组,其浸提液浓度无论是100%还是50%,各时间点的细胞增殖率由高至低依次为MTA组、Roeko Seal组、AHplus组、Cortisomol组;MTA组与Roeko Seal组相比P0.05,其他各组间比较P0.05。结论:MTA与Roeko Seal的细胞毒性最小,AH Plus的细胞毒性居中,Cortisomol的细胞毒性最大,但毒性级均未超过3级。
[Abstract]:Objective: to evaluate the biocompatibility of four root canal sealants. Methods: four root canal sealants were analyzed by X-ray energy spectrometer. The cytotoxic effects of four root canal sealants on L-929 cells were detected by CCK-8 method at 100% and 50% extractant concentrations immediately after solidification and 1 week after solidification. Results: the concentration of two kinds of extractions in the same curing time group was higher than that in the AH Plus group either immediately or 1 week after curing. When the concentration of the extractant was 100, the cell proliferation rate was lower than that of 50% extractant P0.05 at each time point of 4 days, while 100% of the samples of the other three kinds of sealants were compared with the 50% concentration extract. There was no significant difference in cell proliferation rate at each time point (P 0.05). At the same time, the sealants were compared to each other, either immediately or 1 week after curing, The cell proliferation rate of MTA group, Roeko Seal group, Cortisomol group and Roeko Seal group was higher than that of Roeko Seal group (P 0.05). Conclusion the cytotoxicity of Roeko Seal is the least than that of AH Plus. The cytotoxicity of AH Plus is the highest in Cortisomol, but the toxicity level is not more than grade 3.
【作者单位】：解放军第309医院旃坛寺门诊部口腔科;第四军医大学口腔医学院;解放军第二炮兵礼士路门诊部;
【分类号】：R781.05

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