FasL的特异性miRNA阻断舌鳞癌细胞免疫逃逸的研究

发布时间：2018-06-04 10:47

本文选题：FasL + miR-　；参考：《安徽医科大学学报》2017年08期

【摘要】：目的研究特异性微小RNA(miR)-590对舌鳞癌细胞SCC-3 Fas配体(FasL)的表达的影响。方法将合成的miR-590 mimics或miR-590抑制剂转染至SCC-3细胞。通过RT-PCR和Western blot法检测SCC-3中Fas L信使RNA(mRNA)和蛋白的表达;将转染后的SCC-3细胞在顺铂(DDP)5 mg/L处理下,在不同时间段通过MTT法绘制细胞生长曲线,并通过针对Fas L的miR-590的抑制剂从反面验证miR-590的作用。结果 miR-590可以在mRNA和蛋白水平上降低SCC-3细胞Fas L的表达;转染miR-590后SCC-3细胞对于DDP的耐药性降低。结论 miR-590可以有效抑制SCC-3细胞Fas L的表达,降低SCC-3的DDP的耐药性,从而可能降低和减少其对免疫活性细胞的凋亡诱导和杀伤作用,从而阻断肿瘤细胞的免疫逃逸,为舌鳞癌的生物治疗提供了新的靶点。
[Abstract]:Objective to study the effect of specific small RNA(miR)-590 on the expression of SCC-3 Fas ligand in tongue squamous carcinoma cells. Methods the synthesized miR-590 mimics or miR-590 inhibitors were transfected into SCC-3 cells. The expression of Fas L messenger RNA-mRNAs and proteins in SCC-3 was detected by RT-PCR and Western blot methods, and the cell growth curves were drawn by MTT at different time points after the transfected SCC-3 cells were treated with cisplatin DDP5 mg/L. The effect of miR-590 was verified by the inhibition of miR-590 against Fas L. Results miR-590 could decrease the expression of Fas L in SCC-3 cells at the level of mRNA and protein, and decrease the resistance of SCC-3 cells to DDP after transfection of miR-5 90. Conclusion miR-590 can effectively inhibit the expression of Fas L in SCC-3 cells and reduce the drug resistance of SCC-3 DDP, which may reduce the apoptosis induction and killing effect of SCC-3 on immunoreactive cells, thus blocking the immune escape of tumor cells. It provides a new target for the biotherapy of tongue squamous cell carcinoma.
【作者单位】：安徽医科大学口腔医学院;安徽医科大学附属口腔医院安徽省口腔疾病研究中心实验室;
【基金】：安徽省自然科学基金(编号:1508085MH155)
【分类号】：R739.86

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