CCND1、ORAOV1、ERCC1在舌鳞状细胞癌中的表达和临床意义

发布时间：2018-06-06 10:54

本文选题：免疫组化 + 舌鳞状细胞癌　；参考：《口腔医学研究》2015年06期

【摘要】：目的:通过观察CCND1、ORAOV1、ERCC1在舌鳞状细胞癌中的表达情况,探讨CCND1、ORAOV1、ERCC1与舌癌发生、发展的相关性。方法:应用免疫组织化学染色方法检测38例舌鳞状细胞癌组织芯片及4例正常舌组织芯片中CCND1,ORAOV1,ERCC1蛋白表达情况,并分析其与临床病理特征的关系。结果:舌癌组织中CCND1蛋白的阳性表达率明显高于正常舌组织(P0.05),但其过表达水平与正常舌组织相比无统计学差异;CCND1阳性表达率及过表达水平与患者年龄、性别、肿瘤组织学分级及肿瘤有无淋巴结转移之间无明显相关。ORAOV1在舌癌组织中的表达明显升高(P0.05),且与肿瘤有无淋巴结转移之间显著相关(P0.05);ORAOV1在舌癌中的过表达水平与对照组相比无明显差异,但随着肿瘤分化程度的降低ORAOV1的过表达水平显著上升(P0.05)。ERCC1蛋白在舌鳞状细胞癌组织中的阳性表达率及过表达水平均明显低于正常舌组织(P0.05),并均与肿瘤有无淋巴结转移之间显著相关(P0.05);且与高、中分化组相比,低分化组ERCC1蛋白过表达水平上升,但无统计学差异。结论:在舌鳞状细胞癌组织中CCND1和ORAOV1显著高表达,ERCC1表达显著降低,三者的检测对早期诊断舌癌具有指导意义。
[Abstract]:Objective: to investigate the relationship between CCND1ORAOV1 ERCC1 and tongue carcinogenesis by observing the expression of CCND1ORAOV1 ERCC1 in tongue squamous cell carcinoma. Methods: the expression of CCND1 ORAOV1 ERCC1 protein in 38 cases of tongue squamous cell carcinoma and 4 cases of normal tongue tissue microarray was detected by immunohistochemical staining, and the relationship between CCND1 ORAOV1 ERCC1 protein and clinicopathological features was analyzed. Results: the positive expression rate of CCND1 protein in tongue cancer tissue was significantly higher than that in normal tongue tissue (P 0.05), but there was no significant difference between the overexpression level and age and sex of patients. There was no significant correlation between histological grade and lymph node metastasis. The expression of ORAOV1 in tongue carcinoma was significantly increased (P 0.05), and the overexpression level of ORAOV1 in tongue cancer was significantly correlated with lymph node metastasis. There was no significant difference between the two groups. However, with the decrease of tumor differentiation, the overexpression of ORAOV1 increased significantly, and the positive rate and overexpression of ERCC1 protein in tongue squamous cell carcinoma were significantly lower than that in normal tongue tissue. There was a significant correlation between migration and high, The overexpression of ERCC1 protein in the low differentiation group was higher than that in the middle differentiation group, but there was no statistical difference. Conclusion: the expression of CCND1 and ORAOV1 in tongue squamous cell carcinoma is significantly lower than that in control group. The detection of ERCC1 in tongue squamous cell carcinoma may be helpful in the early diagnosis of tongue carcinoma.
【作者单位】：中南大学湘雅三医院口腔科;
【基金】：2014年中南大学中央高校基本科研业务费专项资金资助(2014zzts301)
【分类号】：R739.86

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