微阵列芯片分析成牙骨质细胞矿化过程中microRNA表达谱的差异

发布时间：2018-06-07 00:35

本文选题：成牙骨质细胞 + 矿化　；参考：《口腔医学研究》2017年02期

【摘要】：目的:诱导小鼠成牙骨质细胞矿化,研究在成牙骨质细胞矿化过程中microRNA表达谱的变化。方法:体外培养小鼠成牙骨质细胞系OCCM30,使用抗坏血酸、β-甘油磷酸钠和地塞米松诱导成牙骨质细胞矿化。实时荧光定量PCR(qPCR)检测碱性磷酸酶和骨钙蛋白表达量变化来验证矿化情况。应用miRNA microarray芯片技术,分析成牙骨质细胞矿化过程中miRNA表达谱的变化。QPCR对芯片结果进行验证。结果:成功诱导了成牙骨质细胞的矿化。MiRNA microarray芯片检测发现成牙骨质细胞矿化过程中miRNA表达谱出现明显改变,其中上调的有4个,下调的有39个。QPCR证明芯片结果准确可靠。结论:建立了小鼠成牙骨质细胞矿化的细胞模型,应用miRNA芯片发现小鼠成牙骨质细胞矿化过程中差异表达的miRNA,提示这些miRNA可能参与成牙骨质细胞矿化过程的调控。
[Abstract]:Aim: to study the changes of microRNA expression profile during the mineralization of mouse cementoblasts. Methods: the mouse cementoblast cell line OCCM30 was cultured in vitro. Ascorbic acid, sodium 尾 -glycerophosphate and dexamethasone were used to induce the mineralization of cemental cells. The changes of alkaline phosphatase (ALP) and osteocalcin (BGP) expression were detected by real-time fluorescence quantitative PCR- qPCR to verify mineralization. The changes of miRNA expression profile during the mineralization of cementoblasts were analyzed by miRNA microarray chip technique. QPCR was used to verify the results of the microarray. Results: the mineralization of cementoblasts was successfully induced. MiRNA microarray analysis showed that there were obvious changes in miRNA expression profile during the mineralization of cementoblasts, among which 4 were up-regulated and 39 were down-regulated. The results of microarray were accurate and reliable. Conclusion: a cell model of mouse cementoblast mineralization was established, and miRNA microarray was used to detect the differentially expressed miRNAs during the mineralization of mouse cementoblasts, suggesting that these miRNA may be involved in the regulation of cementoblast mineralization.
【作者单位】：武汉大学口腔医学院口腔基础医学省部共建国家重点实验室培训基地和口腔生物医学教育部重点实验室;武汉大学口腔医院牙周科;
【基金】：国家自然科学基金(编号:81170933,81570946) 湖北省自然科学基金面上项目(2015CFB259)
【分类号】：R781.4

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