局部注射熊果酸对大鼠正畸牙移动距离和牙根吸收的影响

发布时间：2018-06-10 10:24

本文选题：熊果酸 + 正畸牙移动　；参考：《吉林大学》2016年硕士论文

【摘要】：近年来,随着人们生活水平的提高,口腔正畸学也得到蓬勃发展,越来越多的人愿意通过接受正畸治疗的方式,使自己获得自信美观的笑容。但是,在个人因素和环境因素的影响下,正畸治疗也会给一部分患者带来牙根吸收的副作用。因此,开发研制出一种可以预防或治疗牙根吸收的药物,成为了正畸医师们亟待解决的一个问题。牙根吸收的机制与骨吸收的机制十分相似,由破牙骨质细胞发挥重要作用。在受压力侧,牙周组织局部缺血,破牙骨质细胞分化形成并在牙根表面形成吸收陷窝,随着吸收范围的不断扩大,最终导致牙根吸收。蛋白质酪氨酸磷酸酶(Protein-tyrosine phosphatases,PTPs)一直是分子生物学领域的研究热门。有研究表明,其家族成员破骨细胞蛋白质酪氨酸磷酸酶(Osteoclastic protein-tyrosine phosphatase,PTP-oc)在破骨细胞中特异性表达,且对破骨细胞具有正调节作用。目前,已经有学者筛选出PTP-oc的有效抑制剂——熊果酸(Ursolic acid,UA)。既然熊果酸能够以PTP-oc为作用靶点,抑制破骨细胞的活性,那么,熊果酸在正畸牙移动过程中会对牙齿移动距离和牙根吸收发挥怎样的作用呢?在这种研究背景下,作者设计了以下实验,希望可以为牙根吸收的防治提供一定的科学依据。目的:探究局部注射不同剂量的熊果酸对大鼠正畸牙移动距离和牙根吸收的影响。方法:1.建立Wistar大鼠正畸牙移动模型:取96只雄性大鼠随机分为4组,每组24只。在建立大鼠正畸牙移动模型成功后,将熊果酸局部注射于大鼠右侧上颌第一磨牙近中颊侧黏骨膜下,注射剂量分别为0 mmol·L-1(对照组)、0.5 mmol·L-1、1.0 mmol·L-1和2.0 mmol·L-1,每3天1次,每次50μL。分别于加力1 d、3 d、5 d、7 d、10 d、14 d、21 d和28 d后,每组处死3只大鼠。2.制作大鼠上颌精准石膏模型,利用游标卡尺测量并计算各组大鼠右侧上颌第一磨牙的移动距离。3.制备组织标本切片,利用苏木精-伊红(Hematoxylin-Eosin,HE)染色观察大鼠牙根组织的形态学变化。结果:1.4组大鼠的牙齿移动距离均随时间的延长而增大。与对照组相比,0.5 mmol·L-1熊果酸组在加力3 d、7 d、14 d、21 d和28 d后的大鼠牙齿移动距离有统计学意义(P0.05或P0.01);1.0 mmol·L-1和2.0 mmol·L-1熊果酸组在加力1 d后的大鼠牙齿移动距离无统计学意义(P0.05),其他时间点的移动距离与对照组相比较均有统计学意义(P0.01)。0.5 mmol·L-1、1.0 mmol·L-1和2.0 mmol·L-1熊果酸组组间两两比较,在加力5 d、7 d、10 d、14 d、21 d和28 d后大鼠牙齿移动距离均有统计学意义(P0.05或P0.01)。2.大鼠牙根组织形态学变化:随着加力时间的延长,牙根表面开始出现骨吸收陷窝;但随着熊果酸剂量的增加,牙根表面的吸收情况有所缓解。结论:1.局部注射熊果酸可以减小大鼠正畸牙移动的距离。2.伴随注射剂量的增加,大鼠牙齿的移动距离减小。3.熊果酸可以在一定程度上减缓因正畸所致的牙根吸收。
[Abstract]:In recent years, with the improvement of people's living standards, orthodontics has been flourishing. More and more people are willing to receive orthodontic treatment to make themselves smile with confidence and beauty. However, orthodontic treatment can also bring side effects of root resorption in some patients under the influence of personal and environmental factors. Therefore, the development of a drug that can prevent or treat root resorption has become an urgent problem for orthodontists. The mechanism of root resorption is very similar to that of bone resorption, and the osteoclast plays an important role. At the pressure side, periodontal tissue ischemia, osteoclast differentiation and formation of resorption lacuna on the root surface, with the continuous expansion of the absorption range, resulting in root resorption. Protein tyrosine phosphatases (PTPs) has been a hot topic in the field of molecular biology. Some studies have shown that osteoclast protein tyrosine phosphatase (PTP-oco) is specifically expressed in osteoclasts and has a positive regulatory effect on osteoclasts. At present, some scholars have screened out the effective inhibitor of PTP-oc, ursolic acididine UAA. Since ursolic acid can inhibit the activity of osteoclasts by targeting PTP-oc, what effect does ursolic acid play on tooth movement distance and root resorption during orthodontic tooth movement? In this research background, the author designed the following experiments, hoping to provide some scientific basis for the prevention and cure of root resorption. Aim: to investigate the effects of different doses of ursolic acid on the distance and root resorption of orthodontic teeth in rats. Method 1: 1. The orthodontic tooth movement model of Wistar rats was established. 96 male rats were randomly divided into 4 groups with 24 rats in each group. After the establishment of orthodontic tooth movement model in rats, ursolic acid was injected locally into the proximal buccal mucoperiosteal of right maxillary first molar of rats. The dose of ursolic acid was 0 mmol L -1 (control group: 0.5 mmol L -1, 1.0 mmol L -1 and 2.0 mmol L -1, once every 3 days, 50 渭 L each time). Three rats were killed in each group for 21 days and 28 days after adding 1 day, 3 days, 5 days, 7 days, 10 days, 14 days, and 28 days, respectively. A rat maxillary accurate gypsum model was made and the moving distance of the right maxillary first molar was measured and calculated by Vernier caliper. Tissue sections were prepared and the morphological changes of rat root tissue were observed by Hematoxylin-Eosin HEhe staining. Results the distance of tooth movement increased with the extension of time. There was no significant difference in tooth movement distance between 0.5 mmol L-1 ursolic acid group and control group (P 0.05 or P0.01L 1.0 mmol L-1 and 2.0 mmol L-1 ursolic acid group after 1 day of treatment) after 3 days of exposure to ursolic acid for 21 days and 28 days after exposure to ursolic acid. There was no significant difference in the distance of tooth movement between the rats of the ursolic acid group and that of the group of ursolic acid treated with ursolic acid for 1 day. The moving distance at other time points was significantly higher than that in control group (P 0.01). 0.5 mmol L ~ (-1) 1.0 mmol L ~ (-1) and 2.0 mmol L ~ (-1) ursolic acid group. There was significant difference in tooth movement distance of rats after 5 days, 7 days, 10 days, 14 days, 21 days and 28 days, P 0.05 or P 0.01. 2. Morphological changes of root tissue in rats: bone resorption lacunae began to appear on the root surface with the extension of applied time, but with the increase of ursolic acid dose, the resorption of root surface was alleviated. Conclusion 1. Local injection of ursolic acid reduced the distance of orthodontic tooth movement in rats. With the increase of injection dose, the distance of tooth movement in rats decreased by 3. 3. Ursolic acid can slow root resorption due to orthodontics to some extent.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R783.5

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