糖尿病型牙周炎牙龈组织中炎症因子表达及与细胞凋亡关系

发布时间：2018-06-10 17:28

  本文选题：细胞凋亡 + 糖尿病型牙周炎(DAP)　； 参考：《实用口腔医学杂志》2017年04期

【摘要】：目的:探讨糖尿病型牙周炎(diabetes associated periodontitis,DAP)牙龈组织中凋亡细胞发生及与IL-1β和TNFα表达的关系。方法:纳入DAP患者和健康龈(H)受试者各20例,用HE染色和Tunnel法观察牙龈细胞凋亡,透射电镜观察凋亡细胞超微结构;用免疫组化(IHC)检测牙龈组织炎症因子IL-1β和TNFα表达。结果:DAP组牙龈上皮棘细胞层和基底细胞层见明显细胞凋亡,固有层凋亡细胞较少;DAP组牙龈上皮棘细胞和基底细胞凋亡百分率高于H组(P0.01);IHC染色发现DAP组IL-1β和TNFα表达明显高于H组,主要阳性表达细胞为巨噬细胞、浆细胞和淋巴细胞。结论:DAP患者牙龈组织中IL-1β和TNFα在细胞凋亡中起重要作用。
[Abstract]:Objective: to investigate the relationship between the expression of IL-1 尾 and TNF- 伪 in gingival tissues of diabetic periodontitis associated. Methods: the apoptosis of gingival cells was observed by HE staining and tunnel method, and the ultrastructure of apoptotic cells was observed by transmission electron microscope (TEM), and the expression of IL-1 尾 and TNF 伪 in gingival tissue was detected by immunohistochemistry (IHC). Results the apoptotic percentage of gingival epithelial spinous cells and basal cell layers in the control group was higher than that in the H group (P 0.01). The expression of IL-1 尾 and TNF 伪 in DAP group was significantly higher than that in H group. The main positive cells were macrophages, plasma cells and lymphocytes. Conclusion IL-1 尾 and TNF 伪 play an important role in apoptosis in gingival tissues of patients with 鈮，

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