成骨细胞膜片在不同钛表面骨桥蛋白基因和蛋白的表达

发布时间：2018-06-12 10:32

本文选题：钛 + 成骨细胞　；参考：《中国组织工程研究》2017年10期

【摘要】：背景:对钛种植体表面改性是口腔种植骨整合界面研究的重要方法之一,能否通过细胞膜片技术在不同钛表面上实现更好的骨再生效应?目的:初步研究成骨细胞膜片在不同钛表面上的成骨效应。方法:取第3代新生SD大鼠成骨细胞,分别接种于光滑钛表面、喷砂酸蚀钛表面、阳极氧化钛表面,体外构建成骨细胞膜片。接种7,14 d,通过物理刮取法获得成骨细胞膜片,倒置显微镜下观察细胞膜片;采用RT-qP CR检测成骨细胞骨桥蛋白基因的表达,Western blotting检测成骨细胞骨桥蛋白的表达。结果与结论:(1)倒置显微镜下观察:接种第7天,细胞密集,钛片边缘与孔壁间细胞生长良好,呈梭形生长,相互有基质连接,近边缘部偶见细胞生长密集区呈云雾状;接种第14天,细胞膜片中部细胞均匀且重叠生长,形态、界限不清,边缘呈均匀重叠的云雾状;(2)骨桥蛋白表达:接种于喷砂酸蚀钛表面、阳极氧化钛表面的成骨细胞骨桥蛋白表达量均高于接种于光滑钛表面的成骨细胞(P0.05),接种于喷砂酸蚀钛表面与阳极氧化钛表面的成骨细胞骨桥蛋白表达量比较差异无显著性意义;接种于不同材料表面14 d的成骨细胞骨桥蛋白表达均高于对应的7 d表达量(P0.05);(3)结果表明:相比光滑钛片,喷砂酸蚀及阳极氧化钛片对成骨细胞增殖分化有更显著的促进作用。
[Abstract]:Background: surface modification of titanium implants is one of the most important methods in the study of dental implants' integrated interface. Can better bone regeneration effect be achieved on different titanium surfaces by membrane technology? Objective: to study the osteogenic effect of osteoblasts on different titanium surfaces. Methods: osteoblasts of the third generation of SD rats were seeded on smooth titanium surface, sandblasted titanium surface and anodic titanium oxide surface, respectively. Osteoblast membrane was constructed in vitro. The osteoblast membrane was obtained by physical curettage method and the osteopontin gene expression was detected by RT-qP CR and osteopontin expression in osteoblasts was detected by RT-qP CR. Results and conclusion: on the 7th day after inoculation, the cells were dense, and the cells between the edge of titanium slice and the wall of the pore grew well, and the cells grew fusiform and had matrix connections with each other. On the 14th day of inoculation, the cells in the middle of the cell membrane grew evenly and overlapped. The morphology, the boundary was not clear, and the edges were uniform and overlapped in the cloud shape. The expression of osteopontin: inoculated on the surface of sandblasting acid etched titanium. The expression of osteopontin on the surface of anodic titanium oxide was higher than that on the smooth titanium surface, but there was no significant difference between the osteoblasts inoculated on the surface of sandblasting and that on the surface of anodic titanium oxide. The osteopontin expression of osteoblasts inoculated on the surface of different materials for 14 days was higher than that in the corresponding 7 days. The results showed that: compared with smooth titanium tablets, sandblasting and anodic titanium oxide tablets could significantly promote the proliferation and differentiation of osteoblasts.
【作者单位】：贵州医科大学;贵州医科大学附属口腔医院口腔内科;贵州医科大学附属口腔医院口腔修复科;贵州医科大学附属口腔医院口腔正畸学教研室;贵州医科大学附属省人民医院口腔修复科;
【基金】：贵州省科技厅省校合作协议项目(黔科合LH字[2014]7115号) 贵阳市科技计划项目(筑科合同[20141001]39号)~~
【分类号】：R783.6

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