多孔胶原-β-磷酸三钙-硫酸软骨素复合膜的制备与表征

发布时间：2018-06-14 00:49

本文选题：胶原 + β-磷酸三钙　；参考：《材料导报》2017年02期

【摘要】：通过碳化二亚胺(EDC)改性、二次冻干制备多孔胶原-β-磷酸三钙-硫酸软骨素复合膜材料。通过扫描电镜(SEM)、X射线衍射分析仪(XRD)与原子力显微镜(AFM)考察了组分变化与制备过程中复合材料的微观形貌变化,并进一步利用红外、孔隙率、MTT细胞毒性实验等分析手段对复合材料的结构与性能进行了表征。实验结果表明,当胶原盐酸溶解液pH=2,胶原与β-磷酸三钙质量比为1∶2(m(Col)∶m(β-TCP)=1∶2)时,复合材料中β-TCP晶相保持较好,其与胶原之间的排列结合最为均匀紧密。经EDC改性后,SEM与AFM实验均显示了交联后的胶原束明显变大变粗,以一定的方向紧密地排列在一起。XRD图谱显示复合材料中β-TCP特征衍射峰明显。复合材料的孔径为80~90μm,三元膜孔隙率为(90.76±1.28)%,大于纯胶原冻干膜(85.88±0.92)%;红外光谱证实β-TCP中的钙离子与Col上的羧基发生了化学键合,AFM显示β-TCP颗粒能与胶原发生直接联结。复合材料的MTT实验结果为1级,是一种潜在的口腔修复膜材料。
[Abstract]:The porous collagen-尾-tricalcium phosphate-chondroitin sulfate composite membrane was prepared by the modification of carbonized diimide and secondary freeze-drying. The changes of composition and microstructure of composites during preparation were investigated by means of scanning electron microscope (SEM) and atomic force microscope (AFM) by means of X-ray diffraction analyzer (XRD) and atomic force microscope (AFM). The structure and properties of the composites were characterized by MTT cytotoxicity test. The results show that 尾 -TCP crystal phase in the composite remains better when the ratio of collagen to 尾 -tricalcium phosphate is 1: 2 and the mass ratio of collagen to 尾 -tricalcium phosphate is 1: 2m-1: 2. The 尾 -TCP crystal phase in the composite remains better and the binding between 尾 -TCP and collagen is the most uniform and close. The SEM and AFM experiments showed that the cross-linked collagen bundles became larger and thicker, and the 尾 -TCP characteristic diffraction peaks in the composites were clearly shown by the XRD patterns arranged in a certain direction. The pore size of the composite is 80 ~ 90 渭 m and the porosity of the ternary membrane is 90.76 卤1.28, which is larger than that of pure collagen freeze-dried film (85.88 卤0.92). Ir spectra show that the calcium ion in 尾 -TCP has chemical bonding with carboxyl group on Col and AFM shows that 尾 -TCP particles can be directly linked to collagen. The result of MTT test of composite material is grade 1, and it is a potential oral repair membrane material.
【作者单位】：四川大学皮革化学与工程教育部实验室;四川大学生物医学工程技术研究中心;
【基金】：国家自然科学基金(51473001)
【分类号】：R783.1;TB33

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