miR-146a-5p在慢性根尖周炎病损组织中的表达及临床意义

发布时间：2018-06-14 19:32

本文选题：慢性根尖周炎 + miR-a　；参考：《口腔医学研究》2017年06期

【摘要】：目的:比较慢性根尖周炎病损组织和健康牙周膜组织中miR-146a-5p的相对表达水平,探讨miR-146a-5p在慢性根尖周炎发病中的作用及临床意义。方法:收集16例诊断为慢性根尖周炎患牙的根尖周组织作为病例组,8例因正畸拔除的健康牙的牙周膜作为对照组,采用HE染色检测样本的炎症浸润程度,采用实时荧光定量PCR(real-time PCR,RT-PCR)检测miR-146a-5p的表达。所有数据采用SPSS18.0软件包进行统计学分析。结果:HE染色结果可见对照组中无明显炎症细胞浸润,病损组织中可见大量炎症细胞浸润;RT-PCR实验结果显示,病例组与对照组均有miR-146a-5p的表达,且miR-146a-5p在慢性根尖周炎病损组织中的相对表达量明显高于对照组健康牙周膜组织,差异具有统计学意义(P0.05)。结论:miR-146a-5p与慢性根尖周炎的发生发展密切相关。
[Abstract]:Objective: to compare the relative expression of miR-146a-5p in chronic periapical periodontitis and healthy periodontal ligament, and to explore the role and clinical significance of miR-146a-5p in the pathogenesis of chronic periapical periodontitis. Methods: the periapical tissues of 16 patients with chronic periapical periodontitis were collected as the control group. The periodontal membrane of 8 healthy teeth extracted by orthodontic treatment was used as the control group. The degree of inflammatory infiltration of the samples was detected by HE staining. The expression of miR-146a-5p was detected by real-time PCR RT-PCR. All data were analyzed by SPSS 18.0 software package. Results there were no significant inflammatory cell infiltration in the control group and a large number of inflammatory cells infiltrated in the diseased tissue. The results of RT-PCR showed that miR-146a-5p was expressed in both the case group and the control group, and the expression of miR-146a-5p was found in both the case group and the control group. The relative expression of miR-146a-5p in the lesions of chronic periapical periodontitis was significantly higher than that in the healthy periodontal ligament tissues of the control group, and the difference was statistically significant (P 0.05). Conclusion the ratio of miR-146a-5p is closely related to the occurrence and development of chronic periapical periodontitis.
【作者单位】：中国医科大学口腔医学院牙体牙髓病科辽宁省口腔医学研究所牙体牙髓病学研究室辽宁省口腔疾病重点实验室辽宁省口腔疾病转化医学研究中心;
【基金】：沈阳市科学技术基金(编号:F15-199-1-56)
【分类号】：R781.34

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