伊犁黑蜂蜂胶对釉质脱矿和再矿化作用的实验研究

发布时间：2018-06-14 19:44

本文选题：伊犁黑蜂蜂胶 + 牙釉质　；参考：《新疆医科大学》2017年硕士论文

【摘要】：目的:通过体外实验研究伊犁黑蜂蜂胶对牙釉质脱矿和再矿化的作用,评价其防龋效果。方法:应用离体牛切牙制备釉质样本60个,随机分为3组:蜂胶组、氟化钠组(阳性对照)和去离子水组(阴性对照)。分别进行体外脱矿和再矿化实验,应用扫描电镜观察釉质表面形态,采用激光共聚焦扫描显微镜观察釉质脱矿程度,通过测量釉质切片的荧光面积、总荧光量和病损平均荧光量对抑制脱矿和促进再矿化的效果进行量化,采用单因素方差分析进行统计学分析。结果:1.扫描电镜观察示:脱矿后三组都可见釉质表面不平整,但氟化钠组和蜂胶组可见釉质表面颗粒的再附着,去离子水组釉质表面凹凸不平且未见颗粒附着;再矿化后三组釉质表面都有不同程度的形态恢复,氟化钠组的釉质表面较脱矿后更加平整,有许多圆形或椭圆形的细密颗粒沉积,而去离子水组和蜂胶组仍可见釉柱中心发生脱矿所形成的凹坑。2.激光共聚焦扫描显微镜观察示:脱矿后氟化钠组和蜂胶组的荧光面积、总荧光量和平均荧光量均较去离子水组小,差异有统计学意义(P0.05);蜂胶组的荧光面积、总荧光量和平均荧光量均较氟化钠组大,作用虽弱于氟化钠组,但差异不具有统计学意义(P0.05)。再矿化后氟化钠组的荧光面积、总荧光量和平均荧光量均较去离子水组和蜂胶组小,差异有统计学意义(P0.05);蜂胶组的荧光面积、总荧光量和平均荧光量虽均较去离子水组小,但差异不具有统计学意义(P0.05)。结论:伊犁黑蜂蜂胶能够有效抑制牙釉质脱矿,但不具备促进脱矿牙釉质再矿化的作用。
[Abstract]:Objective: to study the effect of Erie bee propolis on demineralization and remineralization of enamel in vitro, and to evaluate the effect of propolis on dental caries. Methods: sixty samples of enamel were prepared from bovine incisors in vitro. They were randomly divided into three groups: propolis group, sodium fluoride group (positive control group) and deionized water group (negative control group). In vitro demineralization and remineralization experiments were carried out respectively. The surface morphology of enamel was observed by scanning electron microscope. The demineralization degree of enamel was observed by laser confocal scanning microscope. The fluorescence area of enamel slice was measured. The total fluorescence and the average fluorescence of the lesion were used to quantify the effect of inhibiting demineralization and promoting the mineralization. The single factor variance analysis was used for statistical analysis. The result is 1: 1. SEM observation showed that the enamel surface was uneven in the three groups after demineralization, but the reattachment of enamel particles could be seen in sodium fluoride group and propolis group, while in deionized water group, the enamel surface was uneven and no particles attached. After remineralization, the enamel surface of the three groups recovered to varying degrees. The enamel surface of the sodium fluoride group was more smooth than that of the demineralized group, and there were a lot of round or elliptical fine particles deposited on the enamel surface. However, deionized water group and propolis formation can still be seen in the center of the enamel post demineralized pit. 2. Laser confocal scanning microscopy showed that the fluorescence area, total fluorescence and average fluorescence of sodium fluoride group and propolis group after demineralization were smaller than those of deionized water group (P 0.05), the fluorescence area of propolis group was lower than that of deionized water group, the fluorescence area of propolis group was lower than that of deionized water group. The total fluorescence quantity and average fluorescence quantity were larger than that of sodium fluoride group, but the difference was not statistically significant (P 0.05), although the effect was weaker than that of sodium fluoride group. The fluorescence area, total fluorescence and average fluorescence of sodium fluoride group after mineralization were smaller than those of deionized water group and propolis group, the difference was statistically significant (P 0.05), the fluorescence area, total fluorescence quantity and average fluorescence quantity of propolis group were smaller than those of deionized water group. But the difference was not statistically significant (P 0.05). Conclusion: Ili bee propolis can effectively inhibit enamel demineralization, but it does not promote demineralization of demineralized enamel.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R781.1

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